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DNALI1 antibody

Anti-DNALI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116217
Anti-DNALI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116217
Anti-DNALI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116217
Anti-DNALI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116217
Anti-DNALI1 antibody used in Western Blot (WB). GTX116217

Cat. No. GTX116217

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DNALI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNALI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619140

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dynein axonemal light intermediate chain 1 , P28 , dJ423B22.5 , hp28

Background

This gene is the human homolog of the Chlamydomonas inner dynein arm gene, p28. The precise function of this gene is not known, however, it is a potential candidate for immotile cilia syndrome (ICS). Ultrastructural defects of the inner dynein arms are seen in patients with ICS. Immotile mutant strains of Chlamydomonas, a biflagellated algae, exhibit similar defects. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNALI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116217

GTX116217 IHC-P Image

DNALI1 antibody detects DNALI1 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DNALI1 antibody (GTX116217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNALI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116217

GTX116217 IHC-P Image

DNALI1 antibody detects DNALI1 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
DNALI1 antibody (GTX116217) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNALI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116217

GTX116217 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using DNALI1(GTX116217) antibody at 1:500 dilution.

Anti-DNALI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX116217

GTX116217 IHC-P Image

DNALI1 antibody detects DNALI1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DNALI1 antibody (GTX116217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNALI1 antibody used in Western Blot (WB). GTX116217

GTX116217 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNALI1 antibody (GTX116217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169