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Nop25 antibody

Anti-Nop25 antibody used in IHC (Paraffin sections) (IHC-P). GTX120303
Anti-Nop25 antibody used in Western Blot (WB). GTX120303
Anti-Nop25 antibody used in IHC (Paraffin sections) (IHC-P). GTX120303
Anti-Nop25 antibody used in Western Blot (WB). GTX120303
Anti-Nop25 antibody used in IHC (Paraffin sections) (IHC-P). GTX120303
Anti-Nop25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120303

Cat. No. GTX120303

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Nop25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Raji , Mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nop25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720141

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleolar protein 12 , Nop25 , dJ37E16.7

Cellular Localization

Nucleus , nucleolus

Background

May bind to 28S rRNA.

Database

Research Area

DATA IMAGES

Anti-Nop25 antibody used in IHC (Paraffin sections) (IHC-P). GTX120303

GTX120303 IHC-P Image

Nop25 antibody detects Nop25 protein at nucleous on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Nop25 antibody (GTX120303) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nop25 antibody used in Western Blot (WB). GTX120303

GTX120303 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX120303 diluted at 1:1000

Anti-Nop25 antibody used in IHC (Paraffin sections) (IHC-P). GTX120303

GTX120303 IHC-P Image

Nop25 antibody detects NOL12 protein at nucleous on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Nop25 antibody (GTX120303) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nop25 antibody used in Western Blot (WB). GTX120303

GTX120303 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX120303 diluted at 1:1000

Anti-Nop25 antibody used in IHC (Paraffin sections) (IHC-P). GTX120303

GTX120303 IHC-P Image

Nop25 antibody detects Nop25 protein at nucleous on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Nop25 antibody (GTX120303) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Nop25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120303

GTX120303 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Nop25(GTX120303) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169