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GCC1 antibody [C1C3]

Anti-GCC1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX120148
Anti-GCC1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120148
Anti-GCC1 antibody [C1C3] used in Western Blot (WB). GTX120148

Cat. No. GTX120148

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rabbit, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721274

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GRIP and coiled-coil domain containing 1 , GCC1P , GCC88

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GCC1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX120148

GTX120148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCC1(GTX120148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GCC1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120148

GTX120148 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using GCC1(GTX120148) antibody at 1:200 dilution.

Anti-GCC1 antibody [C1C3] used in Western Blot (WB). GTX120148

GTX120148 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX120148 diluted at 1:1000

REFERENCE

Application Reference

Bhatt JM et al. Mol Biol Cell 2019; 30 (12) : 1523-1535 Promiscuity of the catalytic Sec7 domain within the guanine nucleotide exchange factor GBF1 in ARF activation, Golgi homeostasis, and effector recruitment.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169