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MUL1 antibody

Orthogonal
Anti-MUL1 antibody used in Western Blot (WB). GTX112673
Anti-MUL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112673
Anti-MUL1 antibody used in Western Blot (WB). GTX112673
Anti-MUL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112673

Cat. No. GTX112673

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all MUL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431 , A431 membrane extract

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MUL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial E3 ubiquitin protein ligase 1 , C1orf166 , GIDE , MAPL , MULAN , RNF218

Cellular Localization

Mitochondrion outer membrane; Multi-pass membrane protein , Peroxisome

Background

E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Database

Research Area

DATA IMAGES

Anti-MUL1 antibody used in Western Blot (WB). GTX112673

GTX112673 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MUL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112673

GTX112673 ICC/IF Image

MUL1 antibody detects MUL1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MUL1 stained by MUL1 antibody (GTX112673) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-MUL1 antibody used in Western Blot (WB). GTX112673

GTX112673 WB Image

Orthogonal

A431 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MUL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112673

GTX112673 IHC-P Image

MUL1 antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
MUL1 antibody (GTX112673) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Farmer T et al. J Cell Sci 2017; 130 (14) : 2359-2370 Control of mitochondrial homeostasis by endocytic regulatory proteins.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169