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TTLL7 antibody [N1N2], N-term

Anti-TTLL7 antibody [N1N2], N-term used in Western Blot (WB). GTX121125

Cat. No. GTX121125

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TTLL7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TTLL7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169439

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin tyrosine ligase like 7

Cellular Localization

Cell projection , cilium , cilium basal body , dendrite , Perikaryon

Background

Polyglutamylase which preferentially modifies beta-tubulin. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step. Required for neurite growth.

Database

Research Area

DATA IMAGES

Anti-TTLL7 antibody [N1N2], N-term used in Western Blot (WB). GTX121125

GTX121125 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TTLL7 antibody [N1N2], N-term (GTX121125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Xiong Z et al. J Exp Med 2019; (Epub) Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169