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LONRF3 antibody [N2C2], Internal

Anti-LONRF3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112150
Anti-LONRF3 antibody [N2C2], Internal used in Western Blot (WB). GTX112150

Cat. No. GTX112150

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LONRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HepG2 , Molt-4

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LONRF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950825

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LON peptidase N-terminal domain and ring finger 3 , RNF127

Background

The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants have been suggested, but their full length natures are not clear. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LONRF3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112150

GTX112150 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using RNF127 (GTX112150) antibody at 1:200 dilution.

Anti-LONRF3 antibody [N2C2], Internal used in Western Blot (WB). GTX112150

GTX112150 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX112150 diluted at 1:1000

REFERENCE

Application Reference

Zeng J et al. Oxid Med Cell Longev 2023; 2023 : 6428579 The Role of the miR-548au-3p/CA12 Axis in Tracheal Chondrogenesis in Congenital Pulmonary Airway Malformations.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169