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ATAD5 antibody

Anti-ATAD5 antibody used in Western Blot (WB). GTX25429

Cat. No. GTX25429

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($589)
See all ATAD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ELISA 1:3000 - 1:15000
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: LDYFRKTSPTNEKT-C conjugated to KLH, corresponding to amino acids 63-76 of Human Elg 1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase family AAA domain containing 5 , C17orf41 , ELG1 , FRAG1

Cellular Localization

Nucleus

Background

Elg1 may be part of an alternative RFC complex involved ensuring replication fidelity.

Database

Research Area

DATA IMAGES

Anti-ATAD5 antibody used in Western Blot (WB). GTX25429

GTX25429 WB Image

Western blot using GeneTex Affinity Purified anti-Elg1 antibody (GTX25429) shows detection of a band ~120 kDa corresponding to human Elg1 in various cell lysates. Lanes contain ~ 5ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589