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NIP30 antibody

Anti-NIP30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122479
Anti-NIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX122479
Anti-NIP30 antibody used in Western Blot (WB). GTX122479

Cat. No. GTX122479

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NIP30 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A549 , H1299

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NIP30. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169902

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

family with sequence similarity 192 member A , C16orf94 , CDA018 , CDA10 , NIP30 , PIP30

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-NIP30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122479

GTX122479 ICC/IF Image

NIP30 antibody detects NIP30 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NIP30 protein stained by NIP30 antibody (GTX122479) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NIP30 antibody used in IHC (Paraffin sections) (IHC-P). GTX122479

GTX122479 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NIP30(GTX122479) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NIP30 antibody used in Western Blot (WB). GTX122479

GTX122479 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
12% SDS PAGE
GTX122479 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169