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CALML3 antibody [N1C3]

Anti-CALML3 antibody [N1C3] used in Western Blot (WB). GTX114954
Anti-CALML3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114954
Anti-CALML3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114954
Anti-CALML3 antibody [N1C3] used in Western Blot (WB). GTX114954

Cat. No. GTX114954

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CALML3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

A431 , mouse liver

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CALML3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620469

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calmodulin like 3 , CLP

Background

May be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinities, to cellular substrates.

Database

Research Area

DATA IMAGES

Anti-CALML3 antibody [N1C3] used in Western Blot (WB). GTX114954

GTX114954 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
15% SDS PAGE
GTX114954 diluted at 1:1000

Anti-CALML3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114954

GTX114954 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CALML3(GTX114954) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CALML3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114954

GTX114954 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CALML3(GTX114954) antibody at 1:200 dilution.

Anti-CALML3 antibody [N1C3] used in Western Blot (WB). GTX114954

GTX114954 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CALML3 antibody [N1C3] (GTX114954) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169