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PABPN1 antibody

Anti-PABPN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101490
Anti-PABPN1 antibody used in Western Blot (WB). GTX101490
Anti-PABPN1 antibody used in Western Blot (WB). GTX101490
Anti-PABPN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101490

Cat. No. GTX101490

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PABPN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

mouse brain , Jurkat

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PABPN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(A) binding protein nuclear 1 , OPMD , PAB2 , PABII , PABP-2 , PABP2

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. An expansion of the trinucleotide (GCG) repeat from normal 6 to 8-13 at the 5' end of the coding region of this gene leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Multiple splice variants have been described but their full-length nature is not known. One splice variant includes introns 1 and 6 but no protein is formed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PABPN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101490

GTX101490 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PABPN1(GTX101490) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PABPN1 antibody used in Western Blot (WB). GTX101490

GTX101490 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX101490 diluted at 1:1000

Anti-PABPN1 antibody used in Western Blot (WB). GTX101490

GTX101490 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
GTX101490 diluted at 1:1000

Anti-PABPN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101490

GTX101490 ICC/IF Image

PABPN1 antibody detects PABPN1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PABPN1 protein stained by PABPN1 antibody (GTX101490) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Matsubara S et al. Neuromuscul. Disord 2016; (Epub) Nuclear inclusions mimicking poly(A)-binding protein nuclear 1 inclusions in a case of inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia with a novel mutation in the valosin-containing protein gene.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169