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ITM2C antibody

Anti-ITM2C antibody used in IHC (Paraffin sections) (IHC-P). GTX116904
Anti-ITM2C antibody used in Western Blot (WB). GTX116904
Anti-ITM2C antibody used in Flow cytometry (FACS). GTX116904

Cat. No. GTX116904

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ITM2C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITM2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624534

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integral membrane protein 2C , BRI3 , BRICD2C , E25 , E25C , ITM3

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.

Database

Research Area

DATA IMAGES

Anti-ITM2C antibody used in IHC (Paraffin sections) (IHC-P). GTX116904

GTX116904 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ITM2C(GTX116904) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ITM2C antibody used in Western Blot (WB). GTX116904

GTX116904 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
GTX116904 diluted at 1:1000

Anti-ITM2C antibody used in Flow cytometry (FACS). GTX116904

GTX116904 FACS Image

The data was published in the journal Int J Mol Sci in 2018.PMID: 30042348

REFERENCE

Application Reference

Trezise S et al. Int J Mol Sci 2018; 19 (8) Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169