APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
|
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
|
Not tested in other applications.
Calculated MW
Positive Control
Unboiled HepG2 membrane extract
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.64 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human TMEM49. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886494
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
vacuole membrane protein 1 , EPG3 , TANGO5 , TMEM49
Cellular Localization
Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein
Background
This gene encodes a transmembrane protein that plays a key regulatory role in the process of autophagy. The ectopic overexpression of the encoded protein in cultured cells triggers autophagy even under nutrient-rich conditions. This gene is overexpressed in pancreatitis affected acinar cells where the encoded protein mediates sequestration and degradation of potentially deleterious activated zymogen granules in a process termed, zymophagy. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
|
GTX131510 WB Image
Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TMEM49 antibody (GTX131510) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
|
REFERENCE
There are currently no references for TMEM49 antibody (GTX131510). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TMEM49 antibody (GTX131510). Be the first to share your experience with this product.
