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ARPC5L antibody [N1C3]

Anti-ARPC5L antibody [N1C3] used in Western Blot (WB). GTX120725
Anti-ARPC5L antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120725
Anti-ARPC5L antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120725

Cat. No. GTX120725

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARPC5L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARPC5L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172404

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin related protein 2/3 complex subunit 5 like , ARC16-2

Cellular Localization

Cytoplasm , cytoskeleton

Background

May function as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.

Database

Research Area

DATA IMAGES

Anti-ARPC5L antibody [N1C3] used in Western Blot (WB). GTX120725

GTX120725 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
15% SDS PAGE
GTX120725 diluted at 1:1000

Anti-ARPC5L antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120725

GTX120725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ARPC5L(GTX120725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARPC5L antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120725

GTX120725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ARPC5L(GTX120725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sadhu L et al. Elife 2023; 12 ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells.
Submit a Reference

REVIEW

There are currently no reviews for ARPC5L antibody [N1C3] (GTX120725). Be the first to share your experience with this product.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169