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ACOX3 antibody

Anti-ACOX3 antibody used in Western Blot (WB). GTX115077
Anti-ACOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115077
Anti-ACOX3 antibody used in Western Blot (WB). GTX115077

Cat. No. GTX115077

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ACOX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A549 , mouse liver

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ACOX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619796

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA oxidase 3, pristanoyl

Cellular Localization

Peroxisome

Background

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ACOX3 antibody used in Western Blot (WB). GTX115077

GTX115077 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX115077 diluted at 1:1000

Anti-ACOX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115077

GTX115077 IHC-P Image

ACOX3 antibody detects ACOX3 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ACOX3 antibody (GTX115077) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACOX3 antibody used in Western Blot (WB). GTX115077

GTX115077 WB Image

ACOX3 antibody detects ACOX3 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
ACOX3 antibody (GTX115077) dilution: 1:500

REFERENCE

Application Reference

Tsai, Kun-chih et al. United States Patent 2016; Application 20160090638 METHODS OF PROGNOSTICALLY CLASSIFYING AND TREATING GLANDULAR CANCERS.
Application : ELISA
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169