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ANKRD32 antibody [HL2311]

Anti-ANKRD32 antibody [HL2311] used in Western Blot (WB). GTX638474
Anti-ANKRD32 antibody [HL2311] used in Western Blot (WB). GTX638474
Anti-ANKRD32 antibody [HL2311] used in Western Blot (WB). GTX638474

Cat. No. GTX638474

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2311

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ANKRD32

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMC5-SMC6 complex localization factor 1 , ANKRD32 , BRCTD1 , BRCTx

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Database

DATA IMAGES

Anti-ANKRD32 antibody [HL2311] used in Western Blot (WB). GTX638474

GTX638474 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ANKRD32 antibody [HL2311] (GTX638474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ANKRD32 antibody [HL2311] used in Western Blot (WB). GTX638474

GTX638474 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ANKRD32 antibody [HL2311] (GTX638474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ANKRD32 antibody [HL2311] used in Western Blot (WB). GTX638474

GTX638474 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ANKRD32 antibody [HL2311] (GTX638474) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

ANKRD32 antibody [HL2311] Cat. No. GTX638474

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse testis tissue
Amount used : 50 μg
Blocking : 5% non fat milk in TBST, 25°C, 0.5-1Hr
Primary Antibody : Undiluted, 4°C, >12Hr
Package List Price ($)
$ 399
$ 169
Custom Conjugations