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FAN antibody [N2C1], Internal

Anti-FAN antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103723
Anti-FAN antibody [N2C1], Internal used in Western Blot (WB). GTX103723

Cat. No. GTX103723

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FAN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

NSMAF transfected 293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FAN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neutral sphingomyelinase activation associated factor , FAN , GRAMD5

Background

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-FAN antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103723

GTX103723 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human T(gastric cancer) , using NSMAF(GTX103723) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FAN antibody [N2C1], Internal used in Western Blot (WB). GTX103723

GTX103723 WB Image

Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody.
A: Non-transfected lysate.
B: NSMAF transfected lysate.
7.5% SDS PAGE
GTX103723 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169