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Calsequestrin 1 antibody

Anti-Calsequestrin 1 antibody used in Western Blot (WB). GTX114304
Anti-Calsequestrin 1 antibody used in Western Blot (WB). GTX114304

Cat. No. GTX114304

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calsequestrin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 , mouse muscle , rat muscle

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calsequestrin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175504

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calsequestrin 1 , CASQ , PDIB1 , VMCQA

Cellular Localization

Sarcoplasmic reticulum lumen , Mitochondrion

Background

The protein encoded by this gene is a mitochondrial calcium-binding protein located in the luminal space of the terminal cisternae of the sarcoplasmic reticulum. The protein binds and putatively stores calcium ions. The protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calsequestrin 1 antibody used in Western Blot (WB). GTX114304

GTX114304 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calsequestrin 1 antibody (GTX114304) diluted at 1:1000.

Anti-Calsequestrin 1 antibody used in Western Blot (WB). GTX114304

GTX114304 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX114304 diluted at 1:1000

REFERENCE

Application Reference

Ferguson DP et al. J Appl Physiol (1985) 2014; 116 (8) : 1057-67 Differential skeletal muscle proteome of high- and low-active mice.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169