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DOT1L antibody [ARC0688]

Anti-DOT1L antibody [ARC0688] used in Western Blot and Immunoprecipitation (WB IP). GTX04640
Anti-DOT1L antibody [ARC0688] used in Western Blot (WB). GTX04640

Cat. No. GTX04640

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 ARC0688

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DOT1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 0.05% BSA, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Dot1L/KMT4 (NP_115871.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DOT1 like histone lysine methyltransferase , DOT1 , KMT4

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-DOT1L antibody [ARC0688] used in Western Blot and Immunoprecipitation (WB IP). GTX04640

GTX04640 WB IP Image

IP analysis of HeLa whole cell lysate using GTX04640 DOT1L antibody [ARC0688].
IP antibody amount : 3μg / 300μg extracts
Dilution : 1:1000

Anti-DOT1L antibody [ARC0688] used in Western Blot (WB). GTX04640

GTX04640 WB Image

WB analysis of various samples using GTX04640 DOT1L antibody [ARC0688].
Loading : 25 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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Package List Price ($)
$ 399