GeneTex

  • Country / Location Selection

United States (US)

DOC2A antibody [N2C3]

Anti-DOC2A antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119085
Anti-DOC2A antibody [N2C3] used in Western Blot (WB). GTX119085

Cat. No. GTX119085

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DOC2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DOC2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617987

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

double C2 domain alpha , Doc2

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Peripheral membrane protein , Cell junction , synapse , synaptosome , Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Cell junction , synapse , synaptosome

Background

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DOC2A antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119085

GTX119085 ICC/IF Image

DOC2A antibody [N2C3] detects DOC2A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DOC2A stained by DOC2A antibody [N2C3] (GTX119085) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DOC2A antibody [N2C3] used in Western Blot (WB). GTX119085

GTX119085 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX119085 diluted at 1:1000

REFERENCE

There are currently no references for DOC2A antibody [N2C3] (GTX119085). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for DOC2A antibody [N2C3] (GTX119085). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169