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CaMK1 (phospho Thr177) antibody

Anti-CaMK1 (phospho Thr177) antibody used in IHC (Paraffin sections) (IHC-P). GTX54970
Anti-CaMK1 (phospho Thr177) antibody used in Western Blot (WB). GTX54970
Anti-CaMK1 (phospho Thr177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54970

Cat. No. GTX54970

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CaMKI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of CaMK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase I , CAMKI

Cellular Localization

Cytoplasm,Nucleus

Background

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CaMK1 (phospho Thr177) antibody used in IHC (Paraffin sections) (IHC-P). GTX54970

GTX54970 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX54970 CaMK1 (phospho Thr177) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-CaMK1 (phospho Thr177) antibody used in Western Blot (WB). GTX54970

GTX54970 WB Image

WB analysis of insulin-treated Jurkat (A) whole cell lysates using GTX54970 CaMK1 (phospho Thr177) antibody.

Anti-CaMK1 (phospho Thr177) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54970

GTX54970 ICC/IF Image

ICC/IF analysis of formalin-fixed Jurkat cells using GTX54970 CaMK1 (phospho Thr177) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399