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BAF53A antibody

Anti-BAF53A antibody used in Western Blot (WB). GTX30021
Anti-BAF53A antibody used in IHC (Paraffin sections) (IHC-P). GTX30021
Anti-BAF53A antibody used in IHC (Paraffin sections) (IHC-P). GTX30021
Anti-BAF53A antibody used in IHC (Paraffin sections) (IHC-P). GTX30021

Cat. No. GTX30021

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BAF53A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6A (NP_004292.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin like 6A , ACTL6 , ARPN-BETA , Arp4 , BAF53A , INO80K

Cellular Localization

Nucleus

Background

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 86 ACTL6A
  • UniProt: O96019 ACTL6A

Research Area

DATA IMAGES

Anti-BAF53A antibody used in Western Blot (WB). GTX30021

GTX30021 WB Image

WB analysis of various sample lysates using GTX30021 BAF53A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BAF53A antibody used in IHC (Paraffin sections) (IHC-P). GTX30021

GTX30021 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX30021 BAF53A antibody.
Dilution : 1:100

Anti-BAF53A antibody used in IHC (Paraffin sections) (IHC-P). GTX30021

GTX30021 IHC-P Image

IHC-P analysis of human placenta tissue using GTX30021 BAF53A antibody.
Dilution : 1:100

Anti-BAF53A antibody used in IHC (Paraffin sections) (IHC-P). GTX30021

GTX30021 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX30021 BAF53A antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399