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Ap3D1 antibody

Anti-Ap3D1 antibody used in Western Blot (WB). GTX23705
Anti-Ap3D1 antibody used in Western Blot (WB). GTX23705

Cat. No. GTX23705

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human
Package
100 μg ($589)
See all Ap3D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IP 1:100
ELISA 1:5000-1:20000
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor related protein complex 3 subunit delta 1 , ADTD , HPS10 , hBLVR

Cellular Localization

Cytoplasm,Golgi apparatus membrane

Background

AP3D1 is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles.

Database

Research Area

DATA IMAGES

Anti-Ap3D1 antibody used in Western Blot (WB). GTX23705

GTX23705 WB Image

Western blot using GeneTex's Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.

Anti-Ap3D1 antibody used in Western Blot (WB). GTX23705

GTX23705 WB Image

WB analysis of HeLa whole cell lysate using GTX23705 Ap3D1 antibody.
Loading : 20 μg
Dilution : 1:500

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589