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MAP7 antibody [C2C3], C-term

Anti-MAP7 antibody [C2C3], C-term used in Western Blot (WB). GTX120907
Anti-MAP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX120907

Cat. No. GTX120907

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MAP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MAP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170884

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 7 , E-MAP-115 , EMAP115

Cellular Localization

Cytoplasm , perinuclear region , Basolateral cell membrane

Background

The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAP7 antibody [C2C3], C-term used in Western Blot (WB). GTX120907

GTX120907 WB Image

Sample (30 μg of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX120907 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX120907

GTX120907 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MAP7(GTX120907) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tymanskyj SR et al. J Neurosci 2019; MAP7 Prevents Axonal Branch Retraction By Creating a Stable Microtubule Boundary to Rescue Polymerization.
Application : ICC/IF
Reactivity : Mouse
Tymanskyj SR et al. Elife 2018; 7 MAP7 regulates axon morphogenesis by recruiting kinesin-1 to microtubules and modulating organelle transport.
Tymanskyj SR et al. J Neurosci 2017; 37 (6) : 1648-1661 MAP7 Regulates Axon Collateral Branch Development in Dorsal Root Ganglion Neurons.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169