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MAP3K13 antibody

Anti-MAP3K13 antibody used in Western Blot (WB). GTX111101
Anti-MAP3K13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111101

Cat. No. GTX111101

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAP3K13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

AsPC1

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAP3K13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885297

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 13 , LZK , MEKK13 , MLK

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MAP3K13 antibody used in Western Blot (WB). GTX111101

GTX111101 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K13 antibody (GTX111101) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP3K13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111101

GTX111101 ICC/IF Image

MAP3K13 antibody detects MAP3K13 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: MAP3K13 protein stained by MAP3K13 antibody (GTX111101) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169