VAPA antibody [N1N3]

Name: VAPA antibody [N1N3]
Brand: GeneTex
SKU: GTX111859
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-VAPA antibody [N1N3] used in Western Blot (WB). GTX111859

Anti-VAPA antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111859

Cat. No. GTX111859

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all VAPA products

Datasheet
Document

View product citations for antibody GTX111859 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

NIH-3T3 , mouse liver , rat liver

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VAPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952537

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VAMP associated protein A , VAP-33 , VAP-A , VAP33 , hVAP-33

Cellular Localization

Cell membrane; Single-pass type IV membrane protein , Endomembrane system; Single-pass type IV membrane protein

Background

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Database

Gene ID: 9218 VAPA
UniProt: Q9P0L0 VAPA

Research Area

DATA IMAGES

GTX111859 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] (GTX111859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111859 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] (GTX111859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111859 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
VAPA antibody
GTX111859 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111859 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] (GTX111859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111859 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using VAPA(GTX111859) antibody at 1:500 dilution.

GTX111859 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using VAPA(GTX111859) antibody at 1:500 dilution.

REFERENCE

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REVIEW