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SYAP1 antibody [N3C3]

Anti-SYAP1 antibody [N3C3] used in Western Blot (WB). GTX120025
Anti-SYAP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120025
Anti-SYAP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120025
Anti-SYAP1 antibody [N3C3] used in Western Blot (WB). GTX120025

Cat. No. GTX120025

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SYAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

293T , Mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SYAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724482

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synapse associated protein 1 , BSTA , PRO3113

Database

DATA IMAGES

Anti-SYAP1 antibody [N3C3] used in Western Blot (WB). GTX120025

GTX120025 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX120025 diluted at 1:500

Anti-SYAP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120025

GTX120025 ICC/IF Image

SYAP1 antibody [N3C3] detects SYAP1 protein at nucleus and Golgi apparatus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SYAP1 protein stained by SYAP1 antibody [N3C3] (GTX120025) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SYAP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120025

GTX120025 IHC-P Image

SYAP1 antibody detects SYAP1 protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. SYAP1 antibody (GTX120025) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SYAP1 antibody [N3C3] used in Western Blot (WB). GTX120025

GTX120025 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX120025 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169