GeneTex

  • Country / Location Selection

United States (US)

JIP1 antibody

Anti-JIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100604
Anti-JIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100604
Anti-JIP1 antibody used in Western Blot (WB). GTX100604

Cat. No. GTX100604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all JIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human JIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950879

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 interacting protein 1 , IB1 , JIP-1 , JIP1 , PRKM8IP

Cellular Localization

Cytoplasm , Cytoplasm , perinuclear region , Nucleus

Background

The protein encoded by this gene is a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-JIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100604

GTX100604 IHC-P Image

JIP1 antibody detects JIP1 protein at cytoplasm on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
JIP1 antibody (GTX100604) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-JIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100604

GTX100604 IHC-P Image

Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using MAPK8IP1(GTX100604) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-JIP1 antibody used in Western Blot (WB). GTX100604

GTX100604 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100604 diluted at 1:1000

REFERENCE

There are currently no references for JIP1 antibody (GTX100604). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for JIP1 antibody (GTX100604). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169