APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Mouse, Rat, Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UCP4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885677
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 25 member 27 , UCP4
Cellular Localization
Mitochondrion inner membrane; Multi-pass membrane protein
Background
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX128022 WB Image
UCP4 antibody detects SLC25A27 protein by Western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
UCP4 antibody (GTX128022) dilution: 1:1000
|
REFERENCE
There are currently no references for UCP4 antibody (GTX128022). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UCP4 antibody (GTX128022). Be the first to share your experience with this product.
