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BZW1 antibody

Anti-BZW1 antibody used in Western Blot (WB). GTX120426
Anti-BZW1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120426
Anti-BZW1 antibody used in Western Blot (WB). GTX120426

Cat. No. GTX120426

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

mouse brain , rat brain , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BZW1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885492

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basic leucine zipper and W2 domains 1 , BZAP45 , Nbla10236

Background

Enhances histone H4 gene transcription but does not seem to bind DNA directly.

Database

DATA IMAGES

Anti-BZW1 antibody used in Western Blot (WB). GTX120426

GTX120426 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BZW1 antibody (GTX120426) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BZW1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120426

GTX120426 ICC/IF Image

BZW1 antibody detects BZW1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BZW1 protein stained by BZW1 antibody (GTX120426) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-BZW1 antibody used in Western Blot (WB). GTX120426

GTX120426 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BZW1 antibody (GTX120426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169