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BRCA1 antibody [17F8] - ChIP grade

KO/KD Orthogonal
Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX70111

Cat. No. GTX70111

Reference 25

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 17F8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all BRCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1 μg/ml
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Product Note

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368627

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111

GTX70111 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111

GTX70111 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

KO/KD

Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

Orthogonal

The data was published in the journal PLoS Pathog in 2016. PMID: 27764250

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

Orthogonal

The data was published in the journal PLoS Pathog in 2016. PMID: 27764250

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

KO/KD Orthogonal

The data was published in the journal PLoS Pathog in 2016. PMID: 27764250

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

KO/KD

The data was published in the journal PLoS Pathog in 2016. PMID: 27764250

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

The data was published in the journal PLoS Pathog in 2016. PMID: 27764250

Anti-BRCA1 antibody [17F8] - ChIP grade used in IHC (Paraffin sections) (IHC-P). GTX70111

GTX70111 IHC-P Image

The data was published in the journal Breast Cancer Res in 2001. PMID: 11250747

REFERENCE

Application Reference

Sun X et al. Nucleic Acids Res 2020; 48 (16) : 9109-9123 NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest.
Huang AC et al. Anticancer Res 2019; 39 (4) : 1839-1847 Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells.
Application : WB
Reactivity : Human
Kong X et al. J Cell Sci 2018; 131 (23) Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair.
Iqbal J et al. PLoS Pathog 2016; 12 (10) : e1005967 histone h2b-ifi16 recognition of nuclear herpesviral genome induces cytoplasmic *-β responses.
Application : WB
Reactivity : Human
Alcaraz Silva B et al. DNA Repair (Amst) 2016; 49 : 43108 Differences in the recruitment of DNA repair proteins at subtelomeric and interstitial I-SceI endonuclease-induced DNA double-strand breaks.
Application : IP
Reactivity : Human
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REVIEW

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Application Reference
Sun X et al. Nucleic Acids Res 2020; 48 (16):9109-9123 NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest.
Huang AC et al. Anticancer Res 2019; 39 (4):1839-1847 Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells.
Application : WB
Reactivity : Human
Kong X et al. J Cell Sci 2018; 131 (23) Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair.
Iqbal J et al. PLoS Pathog 2016; 12 (10):e1005967 histone h2b-ifi16 recognition of nuclear herpesviral genome induces cytoplasmic *-β responses.
Application : WB
Reactivity : Human
Alcaraz Silva B et al. DNA Repair (Amst) 2016; 49:43108 Differences in the recruitment of DNA repair proteins at subtelomeric and interstitial I-SceI endonuclease-induced DNA double-strand breaks.
Application : IP
Reactivity : Human
Johnson BA et al. Virology 2016; 500:22-34 The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.
Application : WB
Reactivity : Human
Shih YL et al. Oncol Rep 2016; 36 (4):2094-100 Casticin induces DNA damage and inhibits DNA repair-associated protein expression in B16F10 mouse melanoma cancer cells.
Application : WB
Reactivity : Mouse
Urban V et al. J Cell Biol 2016; 214 (4):401-15 RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells.
Application : WB
Reactivity : Human
Shang HS et al. Oncol Rep 2016; 36 (4):2207-15 Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.
Dutta D et al. PLoS Pathog 2015; 11 (6):e1005030 brca1 regulates ifi16 mediated nuclear innate sensing of herpes viral dna and subsequent induction of the innate inflammasome and *-β responses.
Application : WB
Reactivity : Human
Buisson R et al. Nucleic Acids Res 2012; 40 (20):10312-23 PALB2 self-interaction controls homologous recombination.
Application : ICC/IF
Reactivity : Human
Bosviel R et al. OMICS 2012; 16 (5):235-44 Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?
Application : IHC-P
Reactivity : Human
Vardi A et al. In Vivo 2010; 24 (4):393-400 Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells.
Oishi H et al. J Biol Chem 2006; 281 (1):43271 An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.
Application : IP
Reactivity : Human
Wada O et al. Oncogene 2004; 23 (35):1497617 BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220.
Application : IP
Reactivity : Human
Bernard-Gallon DJ et al. Anticancer Res 2004; 24 (1):321-4 Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
Application : IHC
Reactivity : Human
Ongusaha PP et al. Oncogene 2003; 22 (24):3749-58 BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
Application : WB
Reactivity : Mouse
Bernard-Gallon DJ et al. Breast Cancer Res 2001; 3 (1):22402 BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Application : IHC
Reactivity : Human
Lu M et al. Oncogene 2000; 19 (54):6351-60 Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms.
Application : WB
de Toledo SM et al. Oncogene 2000; 19 (54):6185-93 ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation.
Application : WB
Reactivity : Human
Arizti P et al. Mol Cell Biol 2000; 20 (20):2027341 Tumor suppressor p53 is required to modulate BRCA1 expression.
Application : WB
Reactivity : Human
Altiok S et al. J Biol Chem 1999; 274 (45):32274-8 Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells.
Application : IP
Reactivity : Human
Bernard-Gallon DJ et al. Int J Cancer 1999; 82 (5):771-3 Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation.
Application : IHC
Reactivity : Human
Yoshikawa K et al. Clin Cancer Res 1999; 5 (6):1249-61 Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases.
Application : IHC
Reactivity : Human
Bernard-Gallon DJ et al. Int J Cancer 1999; 81 (5):839-43 BRCA1 and BRCA2 proteins are expressed in milk fat globules.
Application : IHC
Reactivity : Human
SDS
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Package List Price ($)
$ 399
Custom Conjugations