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SMRT antibody [1542]

Orthogonal
Anti-SMRT antibody [1542] used in Western Blot (WB). GTX70248

Cat. No. GTX70248

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1542

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399)
See all SMRT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

274 kDa. ( Note )

Positive Control

HBL100

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide from the human SMRT protein corresponding to amino acids 994-1005 (ASASDPHREKTQ) coupled to KLH.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_373015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor corepressor 2 , CTG26 , N-CoR2 , SMAP270 , SMRT , SMRTE , SMRTE-tau , TNRC14 , TRAC , TRAC-1 , TRAC1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-SMRT antibody [1542] used in Western Blot (WB). GTX70248

GTX70248 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMRT antibody [1542] (GTX70248) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Liao G et al. J Biol Chem. 2003; 278 (7) : 5052-61 Regulation of androgen receptor activity by the nuclear receptor corepressor SMRT.
Reactivity : Human
Agoulnik IU et al. J Biol Chem 2003; 278 (33) : 31136-48 Repressors of androgen and progesterone receptor action.
Application : IP
Reactivity : Human
Wu X et al. J Biol Chem 2001; 276 (26) : 24177-85 SMRTE inhibits MEF2C transcriptional activation by targeting HDAC4 and 5 to nuclear domains.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399