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Haploid sperm cell-specific antigen antibody [TRA54]

Orthogonal
Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in Western Blot (WB). GTX00716
Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716
Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716

Cat. No. GTX00716

Reference 3

Host

 Rat

Clonality

 Monoclonal

Clone Name

 TRA54

Isotype

 IgM

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot, EM

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all Haploid sperm cell-specific antigen products
PRODUCT

Summary

Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosylation.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Dot Assay dependent
EM Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

adult mouse testis

Purification

Purified IgM
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in Western Blot (WB). GTX00716

GTX00716 WB Image

Orthogonal

WB analysis of various mouse tissue samples using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Dilution : 1 μg/ml
Loading : 100 μg

Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716

GTX00716 IHC-P Image

IHC-P analysis of mouse testis tissue section (8-month) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54]. Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA54 antibody stains reproductive cells.
Dilution : 1:50
Antigen retrieval : 10 mM citrate buffer (pH 6.0) for 15min
Fixation : 4% PFA

Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716

GTX00716 IHC-P Image

IHC-P analysis of mouse testis tissue section (C57BL/6) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Fixation : Bouin's solution

REFERENCE

Clone Reference

Liegel RP et al. Mol Genet Metab Rep. 2014; 1:299-311 Alkylglycerone phosphate synthase (AGPS) deficient mice: models for rhizomelic chondrodysplasia punctate type 3 (RCDP3) malformation syndrome.
Application : IHC-P
Reactivity : Mouse
Arroteia KF et al. PLoS One.? 2014; 9 (8) Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization.
Application : WB, ICC/IF, IP, Dot, EM
Reactivity : Mouse
Deng W et al. Dev Cell. 2002; 2 (6):819-30 miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399