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Rb antibody

Cat. No. GTX20024

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgG1

Application

 WB, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Rat, Chimpanzee
Package
100 μg ($399)
See all Rb products

APPLICATION

Application Note

IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

106 kDa. ( Note )

Positive Control

Tested in a panel of human cell lines and CV-1 cell line (established from monkey kidney epithelium)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 703 and 772 of human RB1.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_372776

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Bartkova J et al. J Pathol 2003; 200 (2) : 149-56 Deregulation of the RB pathway in human testicular germ cell tumours.
Application : IHC
Reactivity : Human
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Package List Price ($)
$ 399
Custom Conjugations