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Glyoxalase I antibody [6F10]

Anti-Glyoxalase I antibody [6F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00705
Anti-Glyoxalase I antibody [6F10] used in Western Blot (WB). GTX00705

Cat. No. GTX00705

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 6F10

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Monkey, Simian
Package
100 μg ($399)
See all Glyoxalase I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1:1000
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant GST-fused mouse glyoxalase I (full length)

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyoxalase 1 , 0610009E22Rik , 1110008E19Rik , 2510049H23Rik , AW550643 , GLY1 , Glo-1 , Glo-1r , Glo-1s , Glo1-r , Glo1-s , Qglo

Database

Research Area

DATA IMAGES

Anti-Glyoxalase I antibody [6F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00705

GTX00705 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00705 Glyoxalase I antibody [6F10].

Anti-Glyoxalase I antibody [6F10] used in Western Blot (WB). GTX00705

GTX00705 WB Image

WB analysis of various samples using GTX00705 Glyoxalase I antibody [6F10]. Mouse GLO1 shows a single band of 27 kDa while human and simian ones show 29 kDa.
Lane 1 : COS-1 whole cell lysate
Lane 2 : L929 whole cell lysate
Lane 3 : HeLa whole cell lysate

REFERENCE

Clone Reference

Hovatta I et al. Nature. 2005; 438 (7068):662-6 Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice.
Chen F et al. Proc Natl Acad Sci U S A. 2004; 101 (20):7687-92 Role for glyoxalase I in Alzheimer's disease.
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399