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pan Cytokeratin antibody [PCK-26]

Anti-pan Cytokeratin antibody [PCK-26] used in Western Blot (WB). GTX26401
Anti-pan Cytokeratin antibody [PCK-26] used in IHC (Paraffin sections) (IHC-P). GTX26401

Cat. No. GTX26401

Reference 19

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PCK-26

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, Dot, Blocking, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Goat, Sheep, Bovine, Cat, Dog, Hamster, Chicken, Guinea pig, Pig, Carp, Lizard, Snake
Package
100 μl ($399)
See all pan Cytokeratin products
PRODUCT

Summary

Pan-Cytokeratin antibody [PCK-26] is a mouse monoclonal antibody that detects multiple cytokeratins (i.e., Type II cytokeratins 1, 5, 6, and 8), a group of intermediate filament proteins present in most human epithelial tissues. This reagent is a broad spectrum antibody that reacts with a variety of normal, reactive, and neoplastic epithelial tissues, as well as simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:300
IHC-Fr Assay dependent
FACS Assay dependent
Dot Assay dependent
Blocking Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

cytokeratin from human epidermis.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385223

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (1,2).

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [PCK-26] used in Western Blot (WB). GTX26401

GTX26401 WB Image

WB analysis of HeLa cell lysate using GTX26401 pan Cytokeratin antibody[PCK-26].
Lane 1 : Antibody dilution 1:300
Lane 2 : Antibody dilution 1:500
Lane 3 : Antibody dilution 1:1000.

Anti-pan Cytokeratin antibody [PCK-26] used in IHC (Paraffin sections) (IHC-P). GTX26401

GTX26401 IHC-P Image

IHC-P analysis of human placenta sections using GTX26401 pan Cytokeratin antibody [PCK-26] at 1:300.

REFERENCE

Application Reference

Nat Genet . 2023; Preneoplastic stromal cells promote BRCA1-mediated breast tumorigenesis
Application : ICC/IF
Campello E et al. Liver Int 2019; (Epub) Changes in plasma circulating microvesicles in patients with HCV-related cirrhosis after treatment with direct-acting antivirals.
Application : FACS
Reactivity : Human
Meyer N et al. Sci Rep 2017; 7 : 45106 Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.
Application : IHC-P
Reactivity : Human
Henderson SE et al. Neoplasia 2016; 18 (12) : 765-774 Suppression of Tumor Growth and Muscle Wasting in a Transgenic Mouse Model of Pancreatic Cancer by the Novel Histone Deacetylase Inhibitor AR-42.
Application : IHC-P
Reactivity : Mouse

Clone Reference

Sobral LM et al. Mol Cancer 2014; stable set knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, * sensitivity and lymph node metastasis in xenograft tumor models.
Application : ICC/IF
Reactivity : Mouse
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REVIEW

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Application Reference
Nat Genet . 2023; Preneoplastic stromal cells promote BRCA1-mediated breast tumorigenesis
Application : ICC/IF
Campello E et al. Liver Int 2019; (Epub) Changes in plasma circulating microvesicles in patients with HCV-related cirrhosis after treatment with direct-acting antivirals.
Application : FACS
Reactivity : Human
Meyer N et al. Sci Rep 2017; 7:45106 Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.
Application : IHC-P
Reactivity : Human
Henderson SE et al. Neoplasia 2016; 18 (12):765-774 Suppression of Tumor Growth and Muscle Wasting in a Transgenic Mouse Model of Pancreatic Cancer by the Novel Histone Deacetylase Inhibitor AR-42.
Application : IHC-P
Reactivity : Mouse
Deshpande P et al. Burns 2013; 39 (6):1170-7 The use of allodermis prepared from Euro skin bank to prepare autologous tissue engineered skin for clinical use.
Application : IHC-P
Reactivity : Human
Clone Reference
Sobral LM et al. Mol Cancer 2014; stable set knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, * sensitivity and lymph node metastasis in xenograft tumor models.
Application : ICC/IF
Reactivity : Mouse
Herrera M et al. Clin Cancer Res 2013; Functional heterogeneity of cancer-associated fibroblasts from human colon tumors shows specific prognostic gene expression signature.
Application : ICC/IF
Reactivity : Human
Okumura N et al. PLoS One 2013; β-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells.
Application : IHC-P
Reactivity : Mouse
Jerke U et al. J Biol Chem. 2013; 288 (18) β2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells.
Application : Blocking
Reactivity : Human
Nelli RK et al. J Virol. 2012; Mammalian innate resistance to highly pathogenic avian influenza H5N1 virus infection is mediated through reduced proinflammation and infectious virus release.
Application : IHC
Reactivity : Human, Pig
Sahu RP et al. Int J Cancer 2012; 131 (7):E1055-66 Mice lacking epidermal PPARγ exhibit a marked augmentation in photocarcinogenesis associated with increased UVB-induced apoptosis, inflammation and barrier dysfunction.
Wojcik E et al. Neurosci Lett. 2011; Characterization of epithelial V-like antigen in human choroid plexus epithelial cells: potential role in CNS immune surveillance.
Reactivity : Human
Sheldon IM et al. PLoS One. 2010; Toll-like receptor 4 mediates the response of epithelial and stromal cells to lipopolysaccharide in the endometrium.
Application : IHC-P
Reactivity : Mouse
Ou J et al. Invest Ophthalmol Vis Sci. 2010; Cytoskeletal and cell adhesion defects in wounded and Pax6+/- corneal epithelia.
Kofler K et al. Pediatr Surg Int 2010; 26 (1):97-104 Fluorescence-activated cell sorting of PCK-26 antigen-positive cells enables selection of ovine esophageal epithelial cells with improved viability on scaffolds for esophagus tissue engineering.
Application : FACS
Reactivity : Sheep
Zhang L et al. Cell Tissue Res 2009; Adam12 plays a role during uterine decidualization in mice
Application : ICC/IF
Reactivity : Mouse
Geisler F et al. Hepatology 2008; Liver-specific inactivation of Notch2, but not Notch1, compromises intrahepatic bile duct development in mice.
Rastaldi MP et al. FASEB J 2006; Glomerular podocytes contain neuron-like functional synaptic vesicles.
Wen J et al. Mol Vis. 2005; Use of superparamagnetic microbeads in tracking subretinal injections.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399