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CFTR antibody [MM13-4]

Cat. No. GTX23270

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MM13-4

Isotype

 IgG1

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
500 μl ($399)
See all CFTR products

APPLICATION

Application Note

Immunofluorescence: assay dependent. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2μg / ml for 60 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.

Calculated MW

168 kDa. ( Note )

Positive Control

Pancreas

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 1387 and 1480 of human CFTR.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_367216

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystic fibrosis transmembrane conductance regulator , ABC35 , ABCC7 , CF , CFTR/MRP , MRP7 , TNR-CFTR , dJ760C5.1

Cellular Localization

Apical cell membrane , Cell membrane , Endoplasmic reticulum membrane , Nucleus

Background

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

Application Reference

He D et al. Biochim Biophys Acta 2008; 1783 (12) : 179517 Repression of CFTR activity in human MMNK-1 cholangiocytes induces sulfotransferase 1E1 expression in co-cultured HepG2 hepatocytes.
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399