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ERCC1 antibody [8F1]

Anti-ERCC1 antibody [8F1] used in IHC (Paraffin sections) (IHC-P). GTX22356

Cat. No. GTX22356

Reference 19

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8F1

Isotype

 IgG2b

Application

 WB, IHC-P, IHC

Reactivity

 Human
Package
500 μl ($399)
See all ERCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100‐1:200
IHC Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BALB/C mice were injected with full length recombinant human ERCC1 protein.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_370420

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 1, endonuclease non-catalytic subunit , COFS4 , RAD10 , UV20

Cellular Localization

Nucleus

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-ERCC1 antibody [8F1] used in IHC (Paraffin sections) (IHC-P). GTX22356

GTX22356 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22356 ERCC1 antibody [8F1].

REFERENCE

Application Reference

Yen-Yun Wang et al. Oncol Lett 2021; 21 (6) : 450 Excision repair cross-complementing group 2 upregulation is a potential predictive biomarker for oral squamous cell carcinoma recurrence.
Application : WB, IHC-P
Reactivity : Human
Mu?oz-Monta?o W et al. BMC Cancer 2021; 21 (1) : 892
Application : IHC-P
Reactivity : Human
Kim SH et al. Cancer Res Treat 2019; (Epub) Identification of Significant Prognostic Tissue Markers Associated with Survival in Upper Urinary Tract Urothelial Carcinoma Patients Treated with Radical Nephroureterectomy: A Retrospective Immunohistochemical Analysis Using Tissue Microarray.
Application : IHC-P
Reactivity : Human
Han JY et al. Cancer Res Treat 2017; 49 (3) : 678-687 ercc1 expression-based randomized phase ii study of */* versus */* in patients with advanced non-small cell lung cancer.
Application : IHC-P
Reactivity : Human
Ho J.A. et al. Pathol Res Pract 2017; (Epub) Prognostic implication of ERCC1 protein expression in resected oropharynx and oral cavity cancer.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
Yen-Yun Wang et al. Oncol Lett 2021; 21 (6):450 Excision repair cross-complementing group 2 upregulation is a potential predictive biomarker for oral squamous cell carcinoma recurrence.
Application : WB, IHC-P
Reactivity : Human
Mu?oz-Monta?o W et al. BMC Cancer 2021; 21 (1):892
Application : IHC-P
Reactivity : Human
Kim SH et al. Cancer Res Treat 2019; (Epub) Identification of Significant Prognostic Tissue Markers Associated with Survival in Upper Urinary Tract Urothelial Carcinoma Patients Treated with Radical Nephroureterectomy: A Retrospective Immunohistochemical Analysis Using Tissue Microarray.
Application : IHC-P
Reactivity : Human
Han JY et al. Cancer Res Treat 2017; 49 (3):678-687 ercc1 expression-based randomized phase ii study of */* versus */* in patients with advanced non-small cell lung cancer.
Application : IHC-P
Reactivity : Human
Ho J.A. et al. Pathol Res Pract 2017; (Epub) Prognostic implication of ERCC1 protein expression in resected oropharynx and oral cavity cancer.
Application : IHC-P
Reactivity : Human
Ahn JS et al. J Clin Oncol 2015; 33 (24):277738 multinational randomized phase iii trial with or without consolidation chemotherapy using * and * after concurrent chemoradiation in inoperable stage iii non-small-cell lung cancer: kcsg-lu05-04.
Application : IHC-P
Reactivity : Human
Okimoto T et al. Anticancer Res 2014; 34 (6):2755-61 Immunohistochemical comparison of biomarker expression in biopsy and surgical specimens of non-small cell lung cancer.
Application : IHC-P
Reactivity : Human
Okimoto T et al. Anticancer Research 2014; 34 (6):2755-2761 Immunohistochemical Comparison of Biomarker Expression in Biopsy and Surgical Specimens of Non-small Cell Lung Cancer.
Application : IHC-P
Reactivity : Human
Yoon YK et al. Cancer Lett 2012; 321 (2):128-36 sunitinib synergizes the antitumor effect of * via modulation of ercc1 expression in models of gastric cancer.
Application : IHC
Reactivity : Human
Tepeli E et al. Mol Biol Rep 2012; 39 (1):335-41 Expression of ERCC1 and its clinicopathological correlations in non-small cell lung cancer.
Application : IHC
Reactivity : Human
Sun JM et al. BMC Cancer 2012; 12:187 ERCC1 as a biomarker for bladder cancer patients likely to benefit from adjuvant chemotherapy.
Application : IHC
Reactivity : Human
Kang CM et al. Pancreas 2012; 41 (3):498-500 In vitro adenosine triphosphate-based chemotherapy response assay (ATP-CRA) in solid pseudopapillary tumor of the pancreas.
Seyhan EC et al. Ann Thorac Cardiovasc Surg 2011; 17 (2):110-7 Prognostic significance of ERCC1 expression in resected non small cell lung carcinoma.
Application : IHC
Reactivity : Human
Won YW et al. Ann Oncol 2011; 22 (2):417-23 a phase ii study of combination chemotherapy with * and * in patients with metastatic or recurrent squamous cell carcinoma of the head and neck.
Application : IHC
Reactivity : Human
Kim SJ et al. Anticancer Res 2010; 30 (12):5245-50 Combination chemotherapy with S-1 and platinum in advanced hepatocellular carcinoma.
Yun J et al. Cancer Res Treat 2010; 42 (2):101-6 predictive value of the ercc1 expression for treatment response and survival in advanced gastric cancer patients receiving *-based first-line chemotherapy.
Application : IHC
Reactivity : Human
Kang CH et al. Lung Cancer 2010; 68 (3):478-83 The prognostic significance of ERCC1, BRCA1, XRCC1, and betaIII-tubulin expression in patients with non-small cell lung cancer treated by platinum- and taxane-based neoadjuvant chemotherapy and surgical resection.
Application : IHC-P
Reactivity : Human
Koh Y et al. Ann Oncol 2009; 20 (8):1414-9 Class III beta-tubulin, but not ERCC1, is a strong predictive and prognostic marker in locally advanced head and neck squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Park S et al. ASCO Meeting Abstracts. 2008; 26:19065 ercc1 and beta tubulin class iii (bt-iii) expression by quantitative immunofluorescence (if) in the responder and non-responder group of patients with advanced non-small-cell lung cancer after 2 cycles of * (p) and * (vn) chemotherapy.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399