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DNA ligase III antibody [1F3]

Orthogonal
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

Cat. No. GTX70143

Reference 25

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F3

Isotype

 IgG1

Application

 WB, ICC/IF, IP, Blocking, PLA

Reactivity

 Human, Mouse, Chicken
Package
100 μl ($399)
See all DNA ligase III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Blocking Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human DNA Ligase III-b.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

RRID

AB_372134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 3 , LIG2 , LIG3alpha

Background

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX70143) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (GTX70143) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The indicated band with observed MW (103 kD) may represent the larger isoform of human DNA Ligase III as reported by Chen J et al and Nash RA et al.

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

Orthogonal

The data was published in the journal PLoS One in 2013. PMID: 23555685

REFERENCE

Application Reference

Rubio-Contreras D et al. Nat Commun 2023; 14 (1) : 6940 TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription.
Nie L et al. bioRxiv 2023; DePARylation is critical for S phase progression and cell survival.
Cong K et al. Mol Cell 2021; 81 (15) : 3128-3144.e7 Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.
Application : WB
Reactivity : Human
Horton JK et al. DNA Repair 2018; 64 : 26-33 XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity.
Julie K. Horton et al. DNA Repair 2017; : Epub ahead of print XRCC1-mediated repair of strand breaks independent of PNKP binding.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Rubio-Contreras D et al. Nat Commun 2023; 14 (1):6940 TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription.
Nie L et al. bioRxiv 2023; DePARylation is critical for S phase progression and cell survival.
Cong K et al. Mol Cell 2021; 81 (15):3128-3144.e7 Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.
Application : WB
Reactivity : Human
Horton JK et al. DNA Repair 2018; 64:26-33 XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity.
Julie K. Horton et al. DNA Repair 2017;:Epub ahead of print XRCC1-mediated repair of strand breaks independent of PNKP binding.
Application : WB
Reactivity : Mouse
Cooper D.J. et al. Stem Cell Research 2016; (Epub) Pluripotent Cells Display Enhanced Resistance to Mutagenesis.
Application : WB
Reactivity : Mouse
Shamanna RA et al. Nat Commun 2016; 7:13785 WRN regulates pathway choice between classical and alternative non-homologous end joining.
Application : WB
Reactivity : Human
Szczesny B et al. Sci Rep 2016; 6:36125 Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics.
Application : PLA
Reactivity : Human
Horton JK et al. Mol Cancer Res 2014; Base excision repair defects invoke hypersensitivity to PARP inhibition.
Application : WB
Reactivity : Mouse
Masaoka A et al. PLoS One 2013; 8 (6):e66801 Interaction between DNA Polymerase β and BRCA1.
Application : WB
Reactivity : Human
Paul K et al. PLoS One 2013; 8 (3):e59505 DNA ligases I and III cooperate in alternative non-homologous end-joining in vertebrates.
Application : WB
Reactivity : Chicken
Moscariello M et al. DNA Repair (Amst) 2013; 12 (11):972-81 Effects of chromatin decondensation on alternative NHEJ.
Application : WB
Reactivity : Mouse
Horton JK et al. DNA Repair (Amst) 2013; 12 (9):774-85 Preventing oxidation of cellular XRCC1 affects PARP-mediated DNA damage responses.
Application : WB
Reactivity : Mouse
Prasad R et al. Nucleic Acids Res 2012; 40 (22):11571-82 Pol β associated complex and base excision repair factors in mouse fibroblasts.
Arakawa H et al. Nucleic Acids Res 2012; 40 (6):2599-610 Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells.
Application : WB
Reactivity : Chicken
Singh SK et al. Int J Radiat Oncol Biol Phys 2011; 79 (2):540-8 Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors.
Application : WB
Reactivity : Mouse
Ebert F et al. Mutat Res 2011; 715 (43102):32-41 Arsenicals affect base excision repair by several mechanisms.
Asagoshi K et al. DNA Repair (Amst) 2010; 9 (2):109-19 DNA polymerase beta-dependent long patch base excision repair in living cells.
Application : WB
Guo Z et al. Nucleic Acids Res 2009; 37 (10):3431-41 Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Application : WB
Reactivity : Mouse
Chen X et al. Cancer Res 2008; 68 (9):3169-77 Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair.
Application : IP
Reactivity : Human
Windhofer F et al. J Cell Physiol 2007; 213 (2):475-83 Low levels of DNA ligases III and IV sufficient for effective NHEJ.
Application : WB
Reactivity : Human
Dong Z et al. Nucleic Acids Res 2006; 34 (20):5721-279 ATM mediates oxidative stress-induced dephosphorylation of DNA ligase IIIalpha.
Application : WB
Reactivity : Human
Okano S et al. Nucleic Acids Res 2005; 33 (1):422-9 Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells.
Application : ICC/IF
Reactivity : Human
Wang H et al. Cancer Res 2005; 65 (10):4020-30 DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Application : Blocking
Reactivity : Human
Leppard JB et al. Mol Cell Biol 2003; 23 (16):5919-27 Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations