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Jab1 antibody [2A10.8]

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203
Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203
Anti-Jab1 antibody [2A10.8] used in Immunohistochemistry (IHC). GTX70203
Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

Cat. No. GTX70203

Reference 11

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A10.8

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399)
See all Jab1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP 0.5 - 1 μg/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , MCF-7 , MDA-MB-231 , Rat liver

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The full-length mouse JAB-1 gene expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_371978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COP9 signalosome subunit 5 , CSN5 , JAB1 , MOV-34 , SGN5

Cellular Localization

Cytoplasm, cytosol , Nucleus

Background

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

GTX70203 WB Image

Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 antibody (GTX70203) diluted at a dilution of 1:500.

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

GTX70203 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX70203 diluted at 1:10000

Anti-Jab1 antibody [2A10.8] used in Immunohistochemistry (IHC). GTX70203

GTX70203 IHC Image

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

GTX70203 WB Image

Sample (50 ug of whole cell lysate)
A: Rat liver
10% SDS PAGE
GTX70203 diluted at 1:1000

REFERENCE

Application Reference

Zhang Z et al. Int J Mol Med 2020; 45 (4) : 1187-1194 Overexpression of IFIT2 Inhibits the Proliferation of Chronic Myeloid Leukemia Cells by Regulating the BCR?ABL/AKT/mTOR Pathway.
Wang S et al. Cancer Lett 2019; 465 : 12-23 MicroRNA-17 acts as a tumor chemosensitizer by targeting JAB1/CSN5 in triple-negative breast cancer.
Application : IHC
Reactivity : Human
Dubiel D et al. Biochim Biophys Acta 2017; (Epub) Cul3 neddylation is crucial for gradual lipid droplet formation during adipogenesis.
Application : WB
Reactivity : Human
Choi HH et al. Oncotarget 2015; 6 (23) : 19721-34 COP1 enhances ubiquitin-mediated degradation of p27Kip1 to promote cancer cell growth.
Application : WB
Reactivity : Human
Jumpertz S et al. Cell Signal 2014; (Epub) The β-catenin E3 ubiquitin ligase SIAH-1 is regulated by CSN5/JAB1 in CRC cells.
Application : IP
Reactivity : Human
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REVIEW

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Application Reference
Zhang Z et al. Int J Mol Med 2020; 45 (4):1187-1194 Overexpression of IFIT2 Inhibits the Proliferation of Chronic Myeloid Leukemia Cells by Regulating the BCR?ABL/AKT/mTOR Pathway.
Wang S et al. Cancer Lett 2019; 465:12-23 MicroRNA-17 acts as a tumor chemosensitizer by targeting JAB1/CSN5 in triple-negative breast cancer.
Application : IHC
Reactivity : Human
Dubiel D et al. Biochim Biophys Acta 2017; (Epub) Cul3 neddylation is crucial for gradual lipid droplet formation during adipogenesis.
Application : WB
Reactivity : Human
Choi HH et al. Oncotarget 2015; 6 (23):19721-34 COP1 enhances ubiquitin-mediated degradation of p27Kip1 to promote cancer cell growth.
Application : WB
Reactivity : Human
Jumpertz S et al. Cell Signal 2014; (Epub) The β-catenin E3 ubiquitin ligase SIAH-1 is regulated by CSN5/JAB1 in CRC cells.
Application : IP
Reactivity : Human
Merk M et al. Proc Natl Acad Sci U S A 2011; 108 (34):E577-85 The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF).
Application : IP
Reactivity : Mouse
McCall CM et al. Mol Cell Biol 2008; 28 (18):5621-33 Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development.
Application : WB
Reactivity : Human
Yun J et al. J Biol Chem 2004; 279 (30):31296-303 Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5.
Application : WB
Reactivity : Human
Doronkin S et al. Development 2002; 129 (21):5053-64 CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint.
Application : WB
Reactivity : Drosophila
Korbonits M et al. J Clin Endocrinol Metab 2002; 87 (6):2635-43 Expression of phosphorylated p27(Kip1) protein and Jun activation domain-binding protein 1 in human pituitary tumors.
Application : IHC
Reactivity : Human
Gemmill RM et al. Oncogene 2002; 21 (22):3507-16 The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations