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MAD1 antibody

Orthogonal
Anti-MAD1 antibody used in Western Blot (WB). GTX109519
Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109519
Anti-MAD1 antibody used in Immunoprecipitation (IP). GTX109519
Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109519
Anti-MAD1 antibody used in Western Blot (WB). GTX109519
Anti-MAD1 antibody used in Western Blot (WB). GTX109519
Anti-MAD1 antibody used in Western Blot (WB). GTX109519
Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109519
Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109519
Anti-MAD1 antibody used in Western Blot (WB). GTX109519

Cat. No. GTX109519

Reference 14

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Potorous tridactylus/Potoroo
Package
100 μl ($399),
25 μl ($169)
See all MAD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Molt-4 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950847

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitotic arrest deficient 1 like 1 , MAD1 , PIG9 , TP53I9 , TXBP181

Cellular Localization

Nucleus , Kinetochore

Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAD1 antibody used in Western Blot (WB). GTX109519

GTX109519 WB Image

MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
MAD1 antibody (GTX109519) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109519

GTX109519 ICC/IF Image

MAD1 antibody detects MAD1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAD1 protein stained by MAD1 antibody (GTX109519) diluted at 1:500.
Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-MAD1 antibody used in Immunoprecipitation (IP). GTX109519

GTX109519 IP Image

Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody (GTX109519).
Western blot analysis was performed using MAD1 antibody (GTX109519).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MAD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109519

GTX109519 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using Mad 1 (GTX109519) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAD1 antibody used in Western Blot (WB). GTX109519

GTX109519 WB Image

MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
MAD1 antibody (GTX109519) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD1 antibody used in Western Blot (WB). GTX109519

GTX109519 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109519 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAD1 antibody used in Western Blot (WB). GTX109519

GTX109519 WB Image

MAD1 antibody detects MAD1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody (GTX109519) diluted by 1:5000.

Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109519

GTX109519 ICC/IF Image

Orthogonal

The data was published in the journal Nat Commun in 2015.PMID: 26620470

Anti-MAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109519

GTX109519 ICC/IF Image

Orthogonal

The data was published in the journal Nat Commun in 2015.PMID: 26620470

Anti-MAD1 antibody used in Western Blot (WB). GTX109519

GTX109519 WB Image

The data was published in the journal Nat Commun in 2016. PMID: 26743940

REFERENCE

Application Reference

Chen C et al. Nat Commun 2023; 14 (1) : 1529 The structural flexibility of MAD1 facilitates the assembly of the Mitotic Checkpoint Complex.
Application : WB
Reactivity : Human
Chihiro Yamada et al. iScience 2022; 25 (2) : 103675 TORC1 inactivation promotes APC/C-dependent mitotic slippage in yeast and human cells.
Application : ICC/IF
Reactivity : Human
Jacob A Herman et al. Elife 2020; 9 chTOG is a conserved mitotic error correction factor.
Application : ICC/IF
Reactivity : Human
Shirnekhi HK et al. J Biol Chem 2020; 295 (43) : 14666-14677 BuGZ facilitates loading of spindle assembly checkpoint proteins to kinetochores in early mitosis.
Application : WB
Reactivity : Human
Shirnekhi HK et al. Mol Biol Cell 2017; 28 (15) : 2035-2041 Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Chen C et al. Nat Commun 2023; 14 (1):1529 The structural flexibility of MAD1 facilitates the assembly of the Mitotic Checkpoint Complex.
Application : WB
Reactivity : Human
Chihiro Yamada et al. iScience 2022; 25 (2):103675 TORC1 inactivation promotes APC/C-dependent mitotic slippage in yeast and human cells.
Application : ICC/IF
Reactivity : Human
Jacob A Herman et al. Elife 2020; 9 chTOG is a conserved mitotic error correction factor.
Application : ICC/IF
Reactivity : Human
Shirnekhi HK et al. J Biol Chem 2020; 295 (43):14666-14677 BuGZ facilitates loading of spindle assembly checkpoint proteins to kinetochores in early mitosis.
Application : WB
Reactivity : Human
Shirnekhi HK et al. Mol Biol Cell 2017; 28 (15):2035-2041 Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.
Application : WB
Reactivity : Human
Tzeng YW et al. Int J Biochem Cell Biol 2017; 94:22-30 LMO7 exerts an effect on mitosis progression and the spindle assembly checkpoint.
Application : WB, ICC/IF
Reactivity : Human
Heasley LR et al. MBoC 2017; (Epub) ‘Wait Anaphase’ Signals Are Not Confined to the Mitotic Spindle.
Application : ICC/IF
Reactivity : Potorous tridactylus/Potoroo
Zhou H et al. Nat Commun 2016; 7:10151 Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Application : WB
Reactivity : Human
Caldas GV et al. Open Biol 2015; 5 (11) The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells.
Application : ICC/IF
Reactivity : Human
Tauchman EC et al. Nat Commun 2015; 6:10036 Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
Akera T et al. Nat Cell Biol 2015; (Epub) Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore.
Application : ICC/IF, IP
Reactivity : Human
Chen Y et al. Oncol Rep 2012; 27 (2):487-91 The spindle checkpoint protein MAD1 regulates the expression of E-cadherin and prevents cell migration.
Application : ChIP assay
Reactivity : Human
Varma D et al. Nat Cell Biol 2012; 14 (6):593-603 Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment.
Application : ICC/IF
Reactivity : Human
Yeh PC et al. J Biol Chem 2012; 287 (15):11704-16 RED, a spindle pole-associated protein, is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint.
Application : IP
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169