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LC3B antibody

Orthogonal
Anti-LC3B antibody used in Western Blot (WB). GTX82986
Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986
Anti-LC3B antibody used in Western Blot (WB). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Western Blot (WB). GTX82986

Cat. No. GTX82986

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, EM

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Primate
Package
100 μl ($449)
See all LC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 0.1 - 2 μg/ml
IHC-P 1:200 - 1:400
IHC-Fr 1:400
EM Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the N-terminal region of the human LC3B protein. [UniProt# Q9GZQ8]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_848127

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm, cytoskeleton

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody used in Western Blot (WB). GTX82986

GTX82986 WB Image

Orthogonal

WB analysis of arsenic trioxide treated U87-MG cell lysate using GTX82986 LC3B antibody.

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986

GTX82986 IHC-P Image

Orthogonal

IHC-P analysis of temozolomide-treated U87-MG xenografts tissue using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Western Blot (WB). GTX82986

GTX82986 WB Image

WB analysis of Neuro2a cell lysate using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82986 LC3B antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:5

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986

GTX82986 IHC-P Image

IHC-P analysis of normal brain and gliobastoma multiform tissue using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82986 LC3B antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1 μg/ml

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

Orthogonal

ICC/IF analysis of temozolomide treated U373-MG cells using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82986 LC3B antibody.
Red : primary antibody
Blue : DAPI

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

Orthogonal

ICC/IF analysis of C2-ceramide treated U373-MG cells using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Western Blot (WB). GTX82986

GTX82986 WB Image

Orthogonal

The data was published in the journal PLoS One in 2016.PMID: 27537685

REFERENCE

Application Reference

G Varga et al. Mol Cell Pediatr 2023; 10 (1) : 19 Phagocytic cell death leads to enhanced release of pro-inflammatory S100A12 in familial Mediterranean fever
Application : WB
Mostafa DK et al. Inflammopharmacology 2022; 30 (6) : 2521-2535
Pan X et al. J Exp Med 2022; 219 (8) Glucosamine amends CNS pathology in mucopolysaccharidosis IIIC mouse expressing misfolded HGSNAT.
Tsai CH et al. Food Chem Toxicol 2021; 154 : 112318 Docosahexaenoic acid promotes the formation of autophagosomes in MCF-7 breast cancer cells through oxidative stress-induced growth inhibitor 1 mediated activation of AMPK/mTOR pathway.
Application : WB
Reactivity : Human
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
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REVIEW

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Application Reference
G Varga et al. Mol Cell Pediatr 2023; 10 (1):19 Phagocytic cell death leads to enhanced release of pro-inflammatory S100A12 in familial Mediterranean fever
Application : WB
Mostafa DK et al. Inflammopharmacology 2022; 30 (6):2521-2535
Pan X et al. J Exp Med 2022; 219 (8) Glucosamine amends CNS pathology in mucopolysaccharidosis IIIC mouse expressing misfolded HGSNAT.
Tsai CH et al. Food Chem Toxicol 2021; 154:112318 Docosahexaenoic acid promotes the formation of autophagosomes in MCF-7 breast cancer cells through oxidative stress-induced growth inhibitor 1 mediated activation of AMPK/mTOR pathway.
Application : WB
Reactivity : Human
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Koenig U et al. Autophagy 2019;:43479 Cell death induced autophagy contributes to terminal differentiation of skin and skin appendages.
Application : WB
Reactivity : Mouse
Sukseree S et al. PLoS One 2016; 11 (8):e0161640 Tyrosinase-Cre-Mediated Deletion of the Autophagy Gene Atg7 Leads to Accumulation of the RPE65 Variant M450 in the Retinal Pigment Epithelium of C57BL/6 Mice.
Application : WB
Reactivity : Mouse
Koenig U et al. Autophagy 2015; 11 (2):298-313 Autophagy facilitates secretion and protects against degeneration of the Harderian gland.
Zhao Y et al. J Invest Dermatol 2013; 133 (6):1629-37 Autophagy is induced by UVA and promotes removal of oxidized phospholipids and protein aggregates in epidermal keratinocytes.
Application : WB
Reactivity : Mouse
Rossiter H et al. J Dermatol Sci 2013; 71 (1):67-75 Epidermal keratinocytes form a functional skin barrier in the absence of Atg7 dependent autophagy.
Application : ICC/IF
Reactivity : Mouse
Rausch V et al. J Pathol 2012; 227 (3):325-35 Autophagy mediates survival of pancreatic tumour-initiating cells in a hypoxic microenvironment.
Application : WB, IHC-Fr
Reactivity : Human
Tomek K et al. Mol Cancer Res 2011; 9 (12):1767-79 Blockade of fatty acid synthase induces ubiquitination and degradation of phosphoinositide-3-kinase signaling proteins in ovarian cancer.
Yang J et al. J Cell Sci 2010; 123 (Pt 6):861-70 GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449