Application Note
For IF: Use at a concentration of 1 - 5 μg/ml. For IP: Use at a concentration of 1 - 5 μg/ml. For WB: Use at a concentration of 1 - 5 μg/ml. Predicted molecular weight: 82 kDa and 20 kDa. Optimal dilutions/concentrations should be determined by the researcher.
Positive Control
SV80 or SV-T2 cells
Product Note
GTX16879 recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen.
Form
Liquid
Buffer
50mM Sodium Phosphate, 0.2% Gelatin
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Full length protein (purified) (SV40).
Purification
Affinity purified with antigen
Conjugation
Unconjugated
RRID
AB_422449
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
Simian virus 40 T antigen ( Large T) , SV40 virus T antigen ( Large T)
Cellular Localization
Nuclear
Background
SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
Research Area