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P Glycoprotein antibody [C219]

Anti-P Glycoprotein antibody [C219] used in Western Blot (WB). GTX23364

Cat. No. GTX23364

Reference 37

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C219

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Carp
Package
250 μl ($429)
See all P Glycoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:50-1:500
ICC/IF Assay dependent
IHC-P ≥1:25
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent

Note :

WB
Recommend using 1% milk in TBST as blocking buffer.

FACS
Cell permeabilization required.
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Observed MW

~140kDa, ~170kDa kDa.

Positive Control

Cell lines or frozen tissue, such as liver or colon

Product Note

Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein. Specific reactivity to human, mouse, rat, dog and primate. Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS, 1% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.

Purification

Purified

Conjugation

Unconjugated

RRID

AB_367204

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily B member 1 , ABC20 , CD243 , CLCS , GP170 , MDR1 , P-GP , PGY1

Cellular Localization

Cell membrane

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Database

Research Area

DATA IMAGES

Anti-P Glycoprotein antibody [C219] used in Western Blot (WB). GTX23364

GTX23364 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

D Miura et al. Fundamental Toxicological Sciences 2023; 10 : 259-264 Titanium dioxide and silica nanoparticles inhibit the efflux of adriamycin in multi-drug resistant L1210/ADM cells
Application : WB
Voirin AC et al. Int J Mol Sci 2023; 24 (5) Loss of Blood-Brain Barrier Integrity in an In Vitro Model Subjected to Intermittent Hypoxia: Is Reversion Possible with a HIF-1α Pathway Inhibitor?
Application : ELISA
Gabriela C Olivera et al. Elife 2021; 10 Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries.
Application : IHC-Fr
Reactivity : Mouse
Levi M et al. Animals (Basel) 2021; 11 (3) High Intrinsic Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Carcinomas Regardless of Immunophenotype and Outcome.
Application : IHC-P
Reactivity : Human
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
D Miura et al. Fundamental Toxicological Sciences 2023; 10:259-264 Titanium dioxide and silica nanoparticles inhibit the efflux of adriamycin in multi-drug resistant L1210/ADM cells
Application : WB
Voirin AC et al. Int J Mol Sci 2023; 24 (5) Loss of Blood-Brain Barrier Integrity in an In Vitro Model Subjected to Intermittent Hypoxia: Is Reversion Possible with a HIF-1α Pathway Inhibitor?
Application : ELISA
Gabriela C Olivera et al. Elife 2021; 10 Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries.
Application : IHC-Fr
Reactivity : Mouse
Levi M et al. Animals (Basel) 2021; 11 (3) High Intrinsic Expression of P-glycoprotein and Breast Cancer Resistance Protein in Canine Mammary Carcinomas Regardless of Immunophenotype and Outcome.
Application : IHC-P
Reactivity : Human
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Reactivity : Human
Zou W et al. Environ Sci Technol 2020; 54 (21):13888-13898 Photo-Oxidative Degradation Mitigated the Developmental Toxicity of Polyamide Microplastics to Zebrafish Larvae by Modulating Macrophage-Triggered Proinflammatory Responses and Apoptosis.
Application : WB
Reactivity : Zebrafish
Deligne C et al. Fluids Barriers CNS 2020; 17 (1):37 Development of a human in vitro blood-brain tumor barrier model of diffuse intrinsic pontine glioma to better understand the chemoresistance.
Application : WB
Reactivity : Human
Yan A et al. J Neurosci 2019; 39 (22):4387-4402 CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2B Adenosine Receptor Signaling.
Kawase A et al. Inflammation 2019; (Epub) Changes in Radixin Expression and Interaction with Efflux Transporters in the Liver of Adjuvant-Induced Arthritic Rats.
Application : WB
Reactivity : Human
Yamaguchi T et al. PLoS One 2019; 14 (9):e0222113 Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models.
Application : WB
Reactivity : Human
Wangta Liu et al. Nucleic Acids 2019; 17:P477-490
Application : WB
Reactivity : Human
Yaguchi Y et al. Mol Pharm 2019; 16 (5):2021-2027 Organic Anion-Transporting Polypeptide 1a4 (Oatp1a4/Slco1a4) at the Blood-Arachnoid Barrier is the Major Pathway of Sulforhodamine-101 Clearance from Cerebrospinal Fluid of Rats.
Application : IHC-Fr
Reactivity : Rat
Lamartiniere Y et al. J Alzheimers Dis 2018; (Epub) ABCA7 Downregulation Modifies Cellular Cholesterol Homeostasis and Decreases Amyloid-β Peptide Efflux in an in vitro Model of the Blood-Brain Barrier.
Application : WB
Reactivity : Mouse
Onozato D et al. Stem Cells Dev 2018; (Epub) Efficient generation of cynomolgus monkey induced pluripotent stem cell-derived intestinal organoids with pharmacokinetic functions.
Application : IHC-Fr
Reactivity : Mouse
Onozato D et al. Drug Metab Dispos 2018; (Epub) Generation of Intestinal Organoids Suitable for Pharmacokinetic Studies from Human Induced Pluripotent Stem Cells.
Application : IHC-Fr
Reactivity : Human
Gao F et al. Basic Clin Pharmacol Toxicol 2018; (Epub) The Sphingosine 1-Phosphate Analogue FTY720 Alleviates Seizure-induced Over-Expression of P-Glycoprotein in Rat Hippocampus.
Reactivity : Rat
Satonaka H et al. Anticancer Res 2017; 37 (11):6071-6077 (-)-epigallocatechin-3-gallate down-regulates *-induced overexpression of p-glycoprotein through the coordinate inhibition of pi3k/akt and mek/erk signaling pathways.
Application : WB
Reactivity : Human
Vellonen KS et al. Pharm Res 2017; (Epub) Disease-Induced Alterations in Brain Drug Transporters in Animal Models of Alzheimer's Disease.
Application : WB
Reactivity : Mouse
Watanabe H et al. Biochem Pharmacol 2017; (Epub) Parathyroid hormone contributes to the down-regulation of cytochrome P450 3A through the cAMP/PI3K/PKC/PKA/NF-κB signaling pathway in secondary hyperparathyroidism.
Application : WB
Reactivity : Human
Chatard M et al. Cell Physiol Biochem 2017; 42 (4):1592-1602 Hydralazine is a Suitable Mimetic Agent of Hypoxia to Study the Impact of Hypoxic Stress on In Vitro Blood-Brain Barrier Model.
Deng L et al. Theranostics 2016; 6 (2):204-18 P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3.
Application : IP
Reactivity : Rat
Ren T et al. Fish Physiol Biochem 2016; (Epub) Toxicity and accumulation of zinc pyrithione in the liver and kidneys of Carassius auratus gibelio: association with P-glycoprotein expression.
Application : IHC-P
Reactivity : Carp
Chatard M et al. PLoS One 2016; 11 (6):e0158010 Hypoxic Stress Induced by * Leads to a Loss of Blood-Brain Barrier Integrity and an Increase in Efflux Transporter Activity.
Application : ELISA
Reactivity : Mouse, Rat
Kanehira Y et al. JDDST 2016; 33:143-148 tumor distribution and anti-tumor effect of * following intrapulmonary administration to mice with metastatic lung tumor.
Application : IHC-Fr
Reactivity : Mouse
Kim DG et al. J Clin Invest 2016; (Epub) A2A adenosine receptor modulates drug efflux transporter P-glycoprotein at the blood-brain barrier.
Application : WB
Reactivity : Human
Togawa N et al. Am J Physiol Cell Physiol 2015; 309 (2):C71-80 Wide expression of type I Na+-phosphate cotransporter 3 (NPT3/SLC17A2), a membrane potential-driven organic anion transporter.
Application : IHC-Fr
Reactivity : Mouse
Yim NH et al. Oncol Rep 2015; 34 (2):1058-64 Induction of apoptotic cell death by betulin in multidrug-resistant human renal carcinoma cells.
Application : WB
Reactivity : Human
Fantappie O et al. J Cell Mol Med 2015; Mitochondria of a human multidrug-resistant hepatocellular carcinoma cell line constitutively express inducible nitric oxide synthase in the inner membrane.
Application : WB
Reactivity : Human
Machado SP et al. Environ Toxicol Pharmacol 2014; 38 (3):720-732 Histopathological lesions, P-glycoprotein and PCNA expression in zebrafish (Danio rerio) liver after a single exposure to diethylnitrosamine.
Application : IHC
Reactivity : Zebrafish
Hu K et al. Environ Toxicol Pharmacol 2014; 37 (2):529-35 Tissue accumulation and toxicity of isothiazolinone in Ctenopharyngodon idellus (grass carp): Association with P-glycoprotein expression and location within tissues
Application : IHC-P
Yokoi K et al. Cancer Lett 2014; 345 (1):48-55 Serum biomarkers for personalization of nanotherapeutics-based therapy in different tumor and organ microenvironments.
Application : IHC-Fr
Reactivity : Mouse
Komori Y et al. Eur J Pharmacol 2014; 724:161-7 ursodeoxycholic acid inhibits overexpression of p-glycoprotein induced by * in hepg2 cells.
Application : WB
Reactivity : Human
Saint-Pol J et al. Brain Res 2013; 1517:43115 Oxysterols decrease apical-to-basolateral transport of As peptides via an ABCB1-mediated process in an in vitro Blood-brain barrier model constituted of bovine brain capillary endothelial cells.
Application : WB
Reactivity : Bovine
Lee YK et al. PLoS One 2013; 8 (11):e82478 galectin-3 silencing inhibits *-induced atp binding cassette transporters and activates the mitochondrial apoptosis pathway via β-catenin/gsk-3β modulation in colorectal carcinoma.
Application : WB
Reactivity : Human
Gong J et al. Eur J Pharmacol 2013; 721 (43103):116-25 Microparticle drug sequestration provides a parallel pathway in the acquisition of cancer drug resistance.
Application : FACS
Reactivity : Human
Yamasaki T et al. Nucl Med Biol 2011; 38 (5):707-14 Evaluation of the P-glycoprotein- and breast cancer resistance protein-mediated brain penetration of 11C-labeled * using small-animal positron emission tomography.
Application : WB
Reactivity : Human
Pelis RM et al. J Pharmacol Exp Ther 2009; 329 (2):479-85 Localization of multidrug resistance-associated protein 2 in the nonpigmented ciliary epithelium of the eye.
Application : WB
Reactivity : Human
SDS
Sodium Azide.pdf
Package List Price ($)
$ 429