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Aquaporin 1 antibody [1/A5F6]

Cat. No. GTX11023

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1/A5F6

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit
Package
50 μg ($399)
See all Aquaporin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/1,000-1/5,000
IHC-P 1/500-1/1,000
IHC-Fr 1/500-1/1,000
ELISA 1/1000-1/20,000
IHC Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids 249-269 of aquaporin 1.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_368040

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aquaporin 1 (Colton blood group) , AQP-CHIP , CHIP28 , CO

Cellular Localization

Cell membrane

Background

This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016]

Database

Research Area

REFERENCE

Application Reference

Takayasu Moroki et al. J Toxicol Pathol 2021; 34 (2) : 161-180 Databases for technical aspects of immunohistochemistry: 2021 update.
Application : IHC
Reactivity : Rat
Terada N et al. Clin Exp Nephrol 2018; (Epub) expression of β-catenin in regenerating renal tubules of *-induced kidney failure in rats.
Application : IHC
Reactivity : Rat
Terada N et al. J Vet Med Sci 2017; (Epub) Immunolocalization of β-catenin, E-cadherin and N-cadherin in neonate and adult rat kidney.
Application : IHC-P
Reactivity : Rat
Kato Y et al. Exp Toxicol Pathol 2017; (Epub) Characterization of pancreatic islet cell tumors and renal tumors induced by a combined treatment of streptozotocin and nicotinamide in male SD rats.
Application : IHC-P
Reactivity : Rat
Fu C et al. J Magn Reson Imaging 2014; Simultaneous changes of magnetic resonance diffusion-weighted imaging and pathological microstructure in locally advanced cervical cancer caused by neoadjuvant chemotherapy.
Application : IHC
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399