CENPA antibody [3-19]

Name: CENPA antibody [3-19]
Brand: GeneTex
SKU: GTX13939
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CENPA antibody [3-19] used in Western Blot (WB). GTX13939

Anti-CENPA antibody [3-19] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13939

Anti-CENPA antibody [3-19] used in IHC (Paraffin sections) (IHC-P). GTX13939

Cat. No. GTX13939

Reference 23

Host	Mouse
Clonality	Monoclonal
Clone Name	3-19
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC, Neutralizing/Inhibition
Reactivity	Human, Chicken, Caenorhabditis elegans
Package	50 μg ($399)

See all CENPA products

View product citations for antibody GTX13939 on CiteAb

APPLICATION

Application Note

ICC: Use at a concentration of 10 μg/ml. IHC: Use at a concentration of 5 μg/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.

Calculated MW

16 kDa. ( Note )

Positive Control

, *BE cells ,

PROPERTIES

Form

Liquid

Buffer

50% Glycerol, PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: PRRRSRKPEAPRRRSPS, corresponding to amino acids 3-19 of Human CENP A.

Purification

Protein A purified
This antibody was purified using Protein A affinity chromatography.

Conjugation

Unconjugated

RRID

AB_369391

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein A , CENP-A , CenH3

Cellular Localization

Nucleus

Background

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]