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NBS1 antibody [1D7]

KO/KD Orthogonal
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

Cat. No. GTX70224

Reference 57

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D7

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NBS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

293T , MCF7 , HeLa (NE) , Raji

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region of the human p95/NBS1 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372445

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nibrin , AT-V1 , AT-V2 , ATV , NBS , NBS1 , P95

Cellular Localization

Nucleus

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70224

GTX70224 ICC/IF Image

NBS1 antibody [1D7] detects NBS1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NBS1 stained by NBS1 antibody [1D7] (GTX70224) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX70224 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 30022091

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Orthogonal

The data was published in the journal Int J Biochem Cell Biol in 2009. PMID: 19782950

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 31913317

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

The data was published in the journal PLoS One in 2014. PMID: 24933654

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

The data was published in the journal PLoS One in 2014. PMID: 24927325

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

The data was published in the journal J Biol Chem in 2013. PMID: 23525106

REFERENCE

Application Reference

McGrail DJ et al. Cell Rep Med 2023; 4 (11) : 101255 Widespread BRCA1/2-independent homologous recombination defects are caused by alterations in RNA-binding proteins.
Grange LJ et al. Nat Commun 2022; 13 (1) : 6664 Pathogenic variants in SLF2 and SMC5 cause segmented chromosomes and mosaic variegated hyperploidy.
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Tsaridou S et al. EMBO Rep 2022; 23 (8) : e54483 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.
Application : ICC/IF
Reactivity : Human
Fangfang Wang et al. Acta Pharm Sin B 2022; 12 (3) : 1339-1350 Chemical screen identifies shikonin as a broad DNA damage response inhibitor that enhances chemotherapy through inhibiting ATM and ATR.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
McGrail DJ et al. Cell Rep Med 2023; 4 (11):101255 Widespread BRCA1/2-independent homologous recombination defects are caused by alterations in RNA-binding proteins.
Grange LJ et al. Nat Commun 2022; 13 (1):6664 Pathogenic variants in SLF2 and SMC5 cause segmented chromosomes and mosaic variegated hyperploidy.
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Tsaridou S et al. EMBO Rep 2022; 23 (8):e54483 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.
Application : ICC/IF
Reactivity : Human
Fangfang Wang et al. Acta Pharm Sin B 2022; 12 (3):1339-1350 Chemical screen identifies shikonin as a broad DNA damage response inhibitor that enhances chemotherapy through inhibiting ATM and ATR.
Application : WB
Reactivity : Human
Boddin J et al. mBio 2022;:e0014422 A Single Amino Acid Switch in the Adenoviral DNA Binding Protein Abrogates Replication Center Formation and Productive Viral Infection.
Abu-Libdeh B et al. J Clin Invest 2022; (Epub) RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1.
Application : WB
Josephine Mun Yee Ko et al. Cancers (Basel) 2021; 13 (18) RAD50 Loss of Function Variants in the Zinc Hook Domain Associated with Higher Risk of Familial Esophageal Squamous Cell Carcinoma.
Application : WB
Reactivity : Human
Sakamoto Y et al. J Radiat Res 2021; 62 (1):25-33 Mitotic cells can repair DNA double-strand breaks via a homology-directed pathway.
Du Y et al. ACS Appl Mater Interfaces 2020; 12 (51):56874-56885 Radiosensitization Effect of AGuIX, a Gadolinium-Based Nanoparticle, in Nonsmall Cell Lung Cancer.
Application : WB
Reactivity : Human
Zhou ZW et al. Nucleic Acids Res 2020; 48 (19):10924-10939 NBS1 interacts with Notch signaling in neuronal homeostasis.
Application : WB
Reactivity : Mouse
Zhou L et al. Signal Transduct Target Ther 2020; 5 (1):80 SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites.
Application : WB
Reactivity : Human
Mooser C et al. Nat Commun 2020; 11 (1):123 Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.
Application : WB
Reactivity : Human
Korsholm LM et al. Nucleic Acids Res 2019; 47 (15):8019-8035 Double-strand breaks in ribosomal RNA genes activate a distinct signaling and chromatin response to facilitate nucleolar restructuring and repair.
Anand R et al. EMBO J 2019; (Epub) NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Application : WB
Reactivity : Human
Sohn SY et al. MBio 2019; 10 (1) Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.
Application : WB
Reactivity : Human
Watanabe S et al. Sci Rep 2018; 8 (1):10888 MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites.
Hu CM et al. FASEB J 2018;:fj201800856R Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation.
Application : ICC/IF
Reactivity : Human
Johnson TE et al. Cell Rep 2018; 24 (6):1471-1483 Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Application : WB
Reactivity : Human
Reynolds JJ et al. Nat Genet 2017; 49 (4):537-549 Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.
Taglialatela A et al. Mol Cell 2017; 68 (2):414-430.e8 Restoration of Replication Fork Stability in BRCA1- and BRCA2-Deficient Cells by Inactivation of SNF2-Family Fork Remodelers.
Application : WB
Reactivity : Human
Li Z et al. Cancer Res 2017; (Epub) Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.
Application : WB
Reactivity : Human
Henrich SM et al. Sci Rep 2017; 7:41663 Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Application : WB, ICC/IF
Reactivity : Human
Fu K et al. Elife 2016; 5 Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation.
Application : WB
Reactivity : Mouse
Sohn SY et al. Proc Natl Acad Sci U S A 2016; 113 (24):6725-30 The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1γ sumoylation and poly-SUMO chain elongation.
Application : WB
Reactivity : Human
Rasmussen RD et al. Mol Oncol 2016; Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma.
Application : WB
Reactivity : Human
Sohn SY et al. J Virol 2015; 89 (3):1744-55 Proteomic analysis of ubiquitin-like posttranslational modifications induced by the adenovirus E4-ORF3 protein.
Application : WB
Reactivity : Human
Koppensteiner R et al. PLoS One 2014; 9 (6):e100041 Effect of MRE11 loss on PARP-inhibitor sensitivity in endometrial cancer in vitro.
Application : WB
Reactivity : Human
Sanjiv K et al. Transl Oncol 2014; 7 (2):256-266.e5 PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
Application : WB
Reactivity : Human
Wei F et al. Oncogene 2014; 0 bmi1 attenuates *-induced g2/m checkpoints via reducing atm activation.
Application : WB
Reactivity : Human
Larsen DH et al. Nat Cell Biol 2014; 16 (8):792-803 The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage.
Application : WB
Reactivity : Human
Lee JH et al. PLoS One 2014; 9 (6):e97969 Direct Activation of ATM by Resveratrol under Oxidizing Conditions.
Application : WB
Reactivity : Human
Ohara M et al. J Radiat Res 2014; Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
Application : WB
Reactivity : Human
O'Sullivan RJ et al. Nat Struct Mol Biol 2014; 21 (2):167-74 Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.
Application : WB
Reactivity : Human
Lee JH et al. J Biol Chem 2013; 288 (18):12840-51 Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
Application : WB
Salewsky B et al. Gene 2013; 519 (2):217-21 Nijmegen breakage syndrome: the clearance pathway for mutant nibrin protein is allele specific.
Sappino AP et al. Oncogenesis 2012; 1:e7 The CEACAM1 tumor suppressor is an ATM and p53-regulated gene required for the induction of cellular senescence by DNA damage.
Application : WB
Reactivity : Human
Oplustilova L et al. Cell Cycle 2012; 11 (20):3837-50 Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Application : WB
Reactivity : Human
Moudry P et al. Cell Death Differ 2012; 19 (5):798-807 Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
E X et al. PLoS Pathog 2011; 7 (5):e1001342 An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus.
Application : ICC/IF
Elakovi? I et al. Brain Res 2011; 1384:22494 Gender-specific response of brain corticosteroid receptors to stress and fluoxetine.
Demogines A et al. PLoS Genet 2010; 6 (10):e1001169 Ancient and recent adaptive evolution of primate non-homologous end joining genes.
Application : WB
Cha H et al. Cancer Res 2010; 70 (10):4112-22 Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
Application : ICC/IF
Reactivity : Human
Mandriota SJ et al. J Biol Chem 2010; 285 (17):13092-106 Ataxia telangiectasia mutated (ATM) inhibition transforms human mammary gland epithelial cells.
Application : WB
Reactivity : Human
Lee JH et al. EMBO J 2010; 29 (3):574-85 53BP1 promotes ATM activity through direct interactions with the MRN complex.
Application : WB
Adzic M et al. Int J Biochem Cell Biol 2009; 41 (11):102848 The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats.
Application : WB
Reactivity : Rat
Zheng L et al. Nucleic Acids Res 2009; 37 (8):2645-57 MRE11 complex links RECQ5 helicase to sites of DNA damage.
Application : WB
Reactivity : Human
Lins S et al. Gene 2009; 447 (1):43441 Clinical variability and expression of the NBN c.657del5 allele in Nijmegen Breakage Syndrome.
Application : WB
Reactivity : Human
Collaco RF et al. Virology 2009; 392 (1):24-33 Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Application : WB
Reactivity : Human
Vago R et al. Cell Cycle 2009; 8 (16):255853 DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories.
Application : ICC/IF
Reactivity : Human
Schroering AG et al. DNA Repair (Amst) 2008; 7 (6):951-69 Rapid induction of chromatin-associated DNA mismatch repair proteins after MNNG treatment.
Bartkova J et al. Mol Oncol 2008; 2 (4):296-316 Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Application : ICC/IF
Reactivity : Human
Spycher C et al. J Cell Biol 2008; 181 (2):227-40 Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin.
Application : ICC/IF
Reactivity : Mouse
den Dulk B et al. DNA Repair (Amst) 2008; 7 (6):858-68 The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4.
Application : WB
Reactivity : Human
Kruger L et al. Carcinogenesis 2007; 28 (1):107-11 Cancer incidence in Nijmegen breakage syndrome is modulated by the amount of a variant NBS protein.
Application : WB
Reactivity : Human
Takemura H et al. J Biol Chem 2006; 281 (41):30814-23 Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Application : WB
Reactivity : Human
Cerosaletti K et al. J Biol Chem 2004; 279 (37):38813-9 Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399