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FOXM1 antibody [C3], C-term

KO/KD Orthogonal Overexpression
Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276
Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276
Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276
Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276
Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276

Cat. No. GTX100276

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FOXM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

FOXM1-transfected 293T

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240833

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box M1 , FKHL16 , FOXM1A , FOXM1B , FOXM1C , HFH-11 , HFH11 , HNF-3 , INS-1 , MPHOSPH2 , MPP-2 , MPP2 , PIG29 , TRIDENT

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276

GTX100276 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term (GTX100276) diluted at 1:5000.

Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276

GTX100276 WB Image

Orthogonal Overexpression

The data was published in the journal Genome Biol in 2015.PMID: 26100407

Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276

GTX100276 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276

GTX100276 WB Image

KO/KD

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-FOXM1 antibody [C3], C-term used in Western Blot (WB). GTX100276

GTX100276 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

REFERENCE

Application Reference

Huang TL et al. Mol Oncol 2022; 16 (13) : 2585-2606 DRP1 contributes to head and neck cancer progression and induces glycolysis through modulated FOXM1/MMP12 axis.
Application : WB
Reactivity : Human
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
Ros S et al. Cancer Cell 2020; 38 (4) : 516-533.e9 Metabolic Imaging Detects Resistance to PI3Kα Inhibition Mediated by Persistent FOXM1 Expression in ER+ Breast Cancer.
Application : ChIP assay
Reactivity : Human
Yang JS et al. Oncol Rep 2018; 39 (1) : 383-391 ITR-284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Otto-Duessel M et al. World J Biol Chem 2017; 8 (2) : 138-150 Identification of neuron selective androgen receptor inhibitors.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Huang TL et al. Mol Oncol 2022; 16 (13):2585-2606 DRP1 contributes to head and neck cancer progression and induces glycolysis through modulated FOXM1/MMP12 axis.
Application : WB
Reactivity : Human
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
Ros S et al. Cancer Cell 2020; 38 (4):516-533.e9 Metabolic Imaging Detects Resistance to PI3Kα Inhibition Mediated by Persistent FOXM1 Expression in ER+ Breast Cancer.
Application : ChIP assay
Reactivity : Human
Yang JS et al. Oncol Rep 2018; 39 (1):383-391 ITR-284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Otto-Duessel M et al. World J Biol Chem 2017; 8 (2):138-150 Identification of neuron selective androgen receptor inhibitors.
Application : WB
Reactivity : Human
Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.
Application : WB
Reactivity : Human
Sanders DA et al. Genome Biol 2015; 16 (Epub):130 FOXM1 binds directly to non-consensus sequences in the human genome.
Application : ChIP assay
Reactivity : Human
Bergamaschi A et al. Breast Cancer Res 2014; 16 (5):436 The forkhead transcription factor FOXM1 promotes endocrine resistance and invasiveness in estrogen receptor-positive breast cancer by expansion of stem-like cancer cells.
Application : ChIP assay
Reactivity : Human
Chen PM et al. Neoplasia 2014; 16 (11):961-71 Up-Regulation of FOXM1 by E6 Oncoprotein through the MZF1/NKX2-1 Axis Is Required for Human Papillomavirus-Associated Tumorigenesis.
Application : WB
Reactivity : Human
Sanders DA et al. Genome Biol 2013; 14 (1):R6 Genome-wide mapping of FOXM1 binding reveals co-binding with estrogen receptor alpha in breast cancer cells.
Application : ChIP assay
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169