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beta Galactosidase antibody

Anti-beta Galactosidase antibody used in Immunohistochemistry (IHC). GTX77365
Anti-beta Galactosidase antibody used in ELISA (ELISA). GTX77365

Cat. No. GTX77365

Reference 15

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IHC

Reactivity

 Mouse, Drosophila, E. coli
Package
100 μg ($399)
See all beta Galactosidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2 - 0.05 ug/ml
ICC/IF 10 - 2 ug/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
IHC 1.0 - 5.0 ug/ml
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Chickens were immunized with purified beta-galactosidase from Escherichia coli. After multiple immunizations in Freunds adjuvant, eggs were collected and delipidated. Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-ga

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_379834

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ECK0341 , JW0335

Database

Research Area

DATA IMAGES

Anti-beta Galactosidase antibody used in Immunohistochemistry (IHC). GTX77365

GTX77365 IHC Image

IHC analysis of immunogen using GTX77365 beta Galactosidase antibody.

Anti-beta Galactosidase antibody used in ELISA (ELISA). GTX77365

GTX77365 ELISA Image

ELISA analysis of immunogen using GTX77365 beta Galactosidase antibody.

REFERENCE

Application Reference

Fan A et al. Bone Res 2023; 11 (1) : 3 Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression.
Martin E et al. Dev Cell 2021; 56 (5) : 687-701.e7 Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.
Emmanuel Martin et al. Dev Cell 2021; 56 (5) : 687-701.e7 Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.
Reactivity : Drosophila
Jonathan M Pojer et al. PLoS Genet 2021; 17 (6) : e1009146 Crumbs and the apical spectrin cytoskeleton regulate R8 cell fate in the Drosophila eye.
Sun J et al. Nat Commun 2021; 12 (1) : 4611 SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts.
Application : IHC-P
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REVIEW

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Application Reference
Fan A et al. Bone Res 2023; 11 (1):3 Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression.
Martin E et al. Dev Cell 2021; 56 (5):687-701.e7 Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.
Emmanuel Martin et al. Dev Cell 2021; 56 (5):687-701.e7 Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.
Reactivity : Drosophila
Jonathan M Pojer et al. PLoS Genet 2021; 17 (6):e1009146 Crumbs and the apical spectrin cytoskeleton regulate R8 cell fate in the Drosophila eye.
Sun J et al. Nat Commun 2021; 12 (1):4611 SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts.
Application : IHC-P
Olinski LE et al. eNeuro 2020; 7 (5) Endogenous Opsin 3 (OPN3) Protein Expression in the Adult Brain Using a Novel OPN3-mCherry Knock-In Mouse Model.
Wei H et al. Cell Rep 2020; 33 (2):108252 Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Application : IHC-Fr
Reactivity : Mouse
Ji G et al. Bone Joint J 2019; 101-B (7_Supple_C):108-114 Vascular endothelial growth factor pathway promotes osseointegration and CD31hiEMCNhi endothelium expansion in a mouse tibial implant model: an animal study.
Application : IHC-Fr
Ghiglione C et al. Development 2018; (Epub) the drosophila * pathway controls profilin expression and dynamic actin-rich protrusions during collective cell migration.
Xu R et al. Nat Med 2018; 24 (6):823-833 Targeting skeletal endothelium to ameliorate bone loss.
Application : IHC-Fr
Agrawal N et al. Cell Metab 2016; 23 (4):675-84 the drosophila tnf eiger is an adipokine that acts on *-producing cells to mediate nutrient response.
Application : ICC/IF
Monier B et al. Nature 2015; 518 (7538):245-8 Apico-basal forces exerted by apoptotic cells drive epithelium folding.
Application : WB
Matusek T et al. Nature 2014; 516 (7529):99-103 The ESCRT machinery regulates the secretion and long-range activity of Hedgehog.
Jouandin P et al. Development 2014; 141 (15):3013-21 Starvation induces FoxO-dependent mitotic-to-endocycle switch pausing during Drosophila oogenesis.
Application : IHC
Gettings M et al. PLoS Biol 2010; 8 (6):e1000390 JNK signalling controls remodelling of the segment boundary through cell reprogramming during Drosophila morphogenesis.
Application : IHC
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399