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VDAC1 antibody [N1C2]

Orthogonal
Anti-VDAC1 antibody [N1C2] used in Western Blot (WB). GTX114187
Anti-VDAC1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114187
Anti-VDAC1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114187
Anti-VDAC1 antibody [N1C2] used in Western Blot (WB). GTX114187

Cat. No. GTX114187

Reference 20
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VDAC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VDAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619641

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

voltage dependent anion channel 1 , PORIN , VDAC-1

Cellular Localization

Mitochondrion outer membrane , Cell membrane

Background

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.

Database

Research Area

DATA IMAGES

Anti-VDAC1 antibody [N1C2] used in Western Blot (WB). GTX114187

GTX114187 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
VDAC1 antibody
GTX114187 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VDAC1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114187

GTX114187 IHC-P Image

VDAC1 antibody [N1C2] detects VDAC1 protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
VDAC1 antibody [N1C2] (GTX114187) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VDAC1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114187

GTX114187 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using VDAC1(GTX114187) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VDAC1 antibody [N1C2] used in Western Blot (WB). GTX114187

GTX114187 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015.PMID: 25658324

REFERENCE

Application Reference

Reshu Saxena et al. Virology 2021; 553 : 135-153 Human neurotropic polyomavirus, JC virus, agnoprotein targets mitochondrion and modulates its functions.
Application : ICC/IF
Reactivity : Human
Ma W et al. Int J Nanomedicine 2020; 15 : 8281-8292 Ameliorative Effect of Sodium Selenite on Silver Nanoparticles-Induced Myocardiocyte Structural Alterations in Rats.
Silvestri E et al. FASEB J 2020; 34 (11) : 15146-15163 Absence of uncoupling protein 3 at thermoneutrality influences brown adipose tissue mitochondrial functionality in mice.
Application : WB
Reactivity : Mouse
Chang YC et al. Toxicol Appl Pharmacol 2020; 401 : 115080 The SMAC mimetic LCL161 is a direct ABCB1/MDR1-ATPase activity modulator and BIRC5/Survivin expression down-regulator in cancer cells.
Application : WB
Reactivity : Human
Ma W et al. Int J Nanomedicine 2020; 15 : 633-645 Silver Nanoparticle Exposure Causes Pulmonary Structural Damage and Mitochondrial Dynamic Imbalance in the Rat: Protective Effects of Sodium Selenite.
Application : WB
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

VDAC1 antibody [N1C2] Cat. No. GTX114187

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Anti-VDAC1 antibody [N1C2] used in Western Blot (WB). GTX114187
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM Surfactin treatment for 120 min.
Anti-VDAC1 antibody [N1C2] used in Western Blot (WB). GTX114187
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : *F05 cells, *F32 cells, *F39 cells, *F90 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Reshu Saxena et al. Virology 2021; 553:135-153 Human neurotropic polyomavirus, JC virus, agnoprotein targets mitochondrion and modulates its functions.
Application : ICC/IF
Reactivity : Human
Ma W et al. Int J Nanomedicine 2020; 15:8281-8292 Ameliorative Effect of Sodium Selenite on Silver Nanoparticles-Induced Myocardiocyte Structural Alterations in Rats.
Silvestri E et al. FASEB J 2020; 34 (11):15146-15163 Absence of uncoupling protein 3 at thermoneutrality influences brown adipose tissue mitochondrial functionality in mice.
Application : WB
Reactivity : Mouse
Chang YC et al. Toxicol Appl Pharmacol 2020; 401:115080 The SMAC mimetic LCL161 is a direct ABCB1/MDR1-ATPase activity modulator and BIRC5/Survivin expression down-regulator in cancer cells.
Application : WB
Reactivity : Human
Ma W et al. Int J Nanomedicine 2020; 15:633-645 Silver Nanoparticle Exposure Causes Pulmonary Structural Damage and Mitochondrial Dynamic Imbalance in the Rat: Protective Effects of Sodium Selenite.
Application : WB
Reactivity : Rat
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Susanne Schuster et al. Journal of Lipid Research 2018; 59 (7) Oxidized linoleic acid metabolites induce liver mitochondrial dysfunction, apoptosis and NLRP3 activation in mice.
Application : WB
Reactivity : Mouse
Lin SC et al. Oncol Rep 2018; 39 (2):703-710 Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.
Application : WB
Garcia-Arroyo F et al. Int. J. Biol. Sci. 2017; 13 (8):961-975 Vasopressin Mediates the Renal Damage Induced by Limited Fructose Rehydration in Recurrently Dehydrated Rats.
Application : WB
Reactivity : Rat
Chen X et al. Redox Biol 2017; 12:987-1003 The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
Application : WB
Reactivity : Rat
Valdez BC et al. Leuk Lymphoma 2017;:43112 the parp inhibitor olaparib enhances the cytotoxicity of combined *, * and * in lymphoma cells.
Application : WB
Reactivity : Human
Eguchi A et al. Hepatology 2016; Extracellular vesicles released by hepatocytes from gastric infusion model of ALD contain a miRNA barcode that can be detected in blood.
Application : WB
Reactivity : Mouse
Chiang MC et al. Exp Cell Res 2016; 347 (2):322-31 * activation of ampk-dependent pathways is neuroprotective in human neural stem cells against amyloid-beta-induced mitochondrial dysfunction.
Application : WB
Reactivity : Human
Yen HC et al. Biochim Biophys Acta 2016; 1860 (9):1864-1876 Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency.
Application : WB
Reactivity : Human
Muzza M et al. Mol Cell Endocrinol 2016; 431:54-61 Oxidative stress and the subcellular localization of the telomerase reverse transcriptase (TERT) in papillary thyroid cancer.
Application : WB
Reactivity : Human
Cho EC et al. Mediators Inflamm 2015; 2015:287345 RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Application : WB
Reactivity : Human
Er-Chieh Cho et al. Mediators of Inflammation 2015; RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress
Application : WB
Reactivity : Human
Lombardi A et al. PLoS One 2015; 10 (2):e0116498 3,5-Diiodo-L-thyronine activates brown adipose tissue thermogenesis in hypothyroid rats.
Application : WB
Reactivity : Rat
Cho EC et al. Oncotarget 2014; 5 (13):4834-44 Tumor suppressor FOXO3 regulates ribonucleotide reductase subunit RRM2B and impacts on survival of cancer patients.
Application : WB
Reactivity : Human
Chuang YL et al. Age (Dordr) 2013; 35 (5):1867-79 Changes in mitochondrial energy utilization in young and old worker honeybees (Apis mellifera).
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production