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HOOK2 antibody

Anti-HOOK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115898
Anti-HOOK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115898
Anti-HOOK2 antibody used in Western Blot (WB). GTX115898
Anti-HOOK2 antibody used in Western Blot (WB). GTX115898
Anti-HOOK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115898
Anti-HOOK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115898
Anti-HOOK2 antibody used in Western Blot (WB). GTX115898

Cat. No. GTX115898

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HOOK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HCT116 , Mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HOOK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hook microtubule tethering protein 2 , HK2

Cellular Localization

Centrosome , Cytoplasm , cytoskeleton

Background

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HOOK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115898

GTX115898 IHC-P Image

HOOK2 antibody detects HOOK2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
HOOK2 antibody (GTX115898) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HOOK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115898

GTX115898 IHC-P Image

HOOK2 antibody detects HOOK2 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
HOOK2 antibody (GTX115898) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HOOK2 antibody used in Western Blot (WB). GTX115898

GTX115898 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX115898 diluted at 1:1000

Anti-HOOK2 antibody used in Western Blot (WB). GTX115898

GTX115898 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX115898 diluted at 1:1000

Anti-HOOK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115898

GTX115898 ICC/IF Image

HOOK2 antibody detects HOOK2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HOOK2 protein stained by HOOK2 antibody (GTX115898) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-HOOK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115898

GTX115898 ICC/IF Image

The data was published in the journal PLoS Genet in 2015. PMID: 25781171

Anti-HOOK2 antibody used in Western Blot (WB). GTX115898

GTX115898 WB Image

The data was published in the journal PLoS Genet in 2015. PMID: 25781171

REFERENCE

Application Reference

Liu Y et al. PLoS Genet 2015; 11 (3) : e1005090 LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility.
Application : IP
Reactivity : Mouse
Onkes W et al. Genes Chromosomes Cancer 2013; 52 (5) : 512-22 Breakpoint characterization of the der(19)t(11;19)(q13;p13) in the ovarian cancer cell line SKOV-3.
Application : WB
Submit a Reference

REVIEW

HOOK2 antibody Cat. No. GTX115898

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-HOOK2 antibody used in Western Blot (WB). GTX115898
Date : Anonymous submitted on 30-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells
Amount used : 10μg
Blocking : 5% skim milk in PBST, 25°C, 0.08Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169